Insertion immediately upstream of the start codon.
Homozygous clones induced in the wing disc survive and grow similarly to wild-type clones for the first two days after induction, but are eliminated by three days after induction.
Homozygous clones induced in the wing disc using the Minute technique form smaller clones over the same time period as wild-type control clones and show defects in epithelial morphology. After 3 days, the mutant cells are largely eliminated from the tissue, being extruded basally from the epithelium and undergoing apoptosis. Extrusion of the mutant cells results in dramatic folds in the epithelium. In smaller, younger clones, the mutant cells appear abnormally short in their apical-basal axis, adopting a more cuboidal morphology than their neighbours. In larger, old clones, abnormally short mutant cells are still visible, but, more dramatically, infolding and extrusion of the mutant cells is seen, creating islands of wild-type epithelium surrounded by mutant cells. A large number of pyknotic nuclei, including apoptotic cells are seen in the mutant clones, with some of these cells appearing to have left the epithelium entirely.
Homozygous clones in the follicular epithelium show defects in epithelial morphology, depending on clone size. In smaller clones, cells appear abnormally contracted along their apical-basal axis. In many larger clones, the cells are rounded up and tend to form double layers. Mutant cells in the follicular epithelium and nurse cells show a striking punctate accumulation of G-actin. The nurse cell membranes break down in the mutant nurse cells, leading to large cells containing multiple nuclei.
Mutant cells are still extruded from the epithelium in homozygous sds22PB1173 clones in the wing disc that are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.
The lethality and mutant phenotypes of sds22PB1173 are reverted by excision of the inserted element.