FlyBase Allele Report: Dmel\Rab7[JF02377]

General Information

Symbol

Dmel\Rab7^JF02377

Species

D. melanogaster

Name

FlyBase ID

FBal0220741

Feature type

allele

Associated gene

Dmel\Rab7

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02377}

Also Known As

UAS-Rab7-RNAi, Rab7 RNAi, UAS-rab7 RNAi, Rab7^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

(Perkins et al., 2009.3.31)

Progenitor genotype

Carried in construct

P{TRiP.JF02377}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.3.31)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02377

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^en-e16E

(Han et al., 2014)

Phenotype Manifest In

extracellular exosome, with Scer\GAL4^dsx.KI

(Corrigan et al., 2014)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^en-e16E

(Han et al., 2014)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Corrigan et al., 2014)

Detailed Description

Statement

Reference

The expression of Rab7^JF02377 under the control of Scer\GAL4^Cg.PA does not induce an accumulation of autophagosome-like structures (Atg8a-positive) in the early third instar larval fat body, as compared to controls.

(Liu et al., 2018)

Animals expressing Rab7^JF02377 under the control of Scer\GAL4^grh.D4 are viable, no obvious defects in larval dorsal trunk elongation and airway morphology are observed and the larvae are of comparable size to age-matched controls.

(Wingen et al., 2017)

Expression of Rab7^JF02377 RNAi under the control of Scer\GAL4^GMR.PFa does not perturb the structure or appearance of the adult eye compared to controls.

(Ruan et al., 2016)

Expression of Rab7^JF02377 in secondary cells under the control of Scer\GAL4^dsx.KI reduces exosome secretion into the accessory gland lumen compared to controls.

(Corrigan et al., 2014)

Expression of Rab7^JF02377 (co-expressed with Dcr-2^Scer\UAS.cDa) in posterior epidermal cells under the control of Scer\GAL4^en-e16E partially prevents phagocytic clearance of ddaC neuron dendrite debris following pupal stage dendritic pruning. In contrast, when larval dendrites are injured (severed with an infrared laser) dendritic debris is completely cleared.

(Han et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rab7^JF02377, Scer\GAL4^GMR.PFa is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Suppressor of

Statement

Reference

Rab7^JF02377, Scer\GAL4^ptc.PU is a suppressor | partially of increased mortality during development phenotype of Hsap\KIF5B::Hsap\RET^UAS.cDa, Scer\GAL4^ptc.PU

(Das and Cagan, 2017)

NOT Suppressor of

Statement

Reference

Rab7^JF02377, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Other

Statement

Reference

Hsap\KIF5B::Hsap\RET^UAS.cDa, Rab7^JF02377, Scer\GAL4^ptc.PU has partially lethal - majority die phenotype

(Das and Cagan, 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab7^JF02377, Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Rab7^JF02377, Scer\GAL4^Cg.PA is a non-suppressor of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Rab7^JF02377, Scer\GAL4^Cg.PA is a non-suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab7^JF02377 RNAi in animals expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa does not perturb the localization of egr to early endosomes in third instar larval eye disc.

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is exacerbated further by co-expression of Rab7^JF02377 RNAi resulting in a 'no eye' phenotype with necrosis-like tissue present.

(Ruan et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27051

y¹ v¹; P{TRiP.JF02377}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rab7^JF02377

(Hertenstein et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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