FlyBase Allele Report: Dmel\par-1[RNAi.UAS.cMDa]

General Information

Symbol

Dmel\par-1^{RNAi.UAS.cMDa}

Species

D. melanogaster

Name

FlyBase ID

FBal0220462

Feature type

allele

Associated gene

Dmel\par-1

Associated Insertion(s)

Carried in Construct

P{UAS-par-1.RNAi.MD}

Also Known As

UAS-PAR-1 RNAi

Key Links

Transgenic product class

RNAi_reagent

(McDonald et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(McDonald et al., 2008)

Mutagen

in vitro construct

(McDonald et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-par-1.RNAi.MD}

Cytology

Description

UAS regulatory sequences drive expression of a construct that produces par-1 dsRNA.

(McDonald et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

par-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa, milt^GD8116

(Iijima-Ando et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell, with Scer\GAL4^c306

(McDonald et al., 2008)

border follicle cell, with Scer\GAL4^c306, par-1^27C1

(McDonald et al., 2008)

border follicle cell, with Scer\GAL4^c306, par-1^70D3

(McDonald et al., 2008)

border follicle cell, with Scer\GAL4^c306, par-1^Δ-16

(McDonald et al., 2008)

Detailed Description

Statement

Reference

Expression of par-1^{dsRNA.Scer\UAS.cMDa} under the control of Scer\GAL4^c306 disrupts border follicle cell migration, such that only approximately 60% of border cells complete migration. The severity of the border cell migration defects are increased if the flies are also heterozygous for par-1^27C1, par-1^Δ-16 or par-1^70D3.

Border follicle cells expressing par-1^{dsRNA.Scer\UAS.cMDa} under the control of Scer\GAL4^c306 extend protrusions as occurs in wild-type border cells, but in contrast to wild-type cells, where the protrusions are extended mainly in the forward direction (in the direction of migration), in the mutant cells forward protrusions are decreased at the expense of backward and lateral protrusions.

(McDonald et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

par-1^{RNAi.UAS.cMDa}, Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF, milt^GD8116

(Iijima-Ando et al., 2012)

par-1^{RNAi.UAS.cMDa}/Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^GMR.PF, milt^GD8116

(Iijima-Ando et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

par-1^{RNAi.UAS.cMDa}, Scer\GAL4^GMR.PF is a suppressor of lamina phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF, milt^GD8116

(Iijima-Ando et al., 2012)

par-1^{RNAi.UAS.cMDa}/Scer\GAL4^GMR.PF is a suppressor of lamina phenotype of Scer\GAL4^GMR.PF, milt^GD8116

(Iijima-Ando et al., 2012)

par-1^{RNAi.UAS.cMDa}, Scer\GAL4^GMR.PF is a suppressor of autophagosome phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

par-1^{RNAi.UAS.cMDa}, Scer\GAL4^GMR.PF is a suppressor of multivesicular body phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of par-1^{dsRNA.Scer\UAS.cMDa} suppresses the neurodegeneration seen in the lamina of 30 day old flies when milt^GD8116 is expressed under the control of Scer\GAL4^GMR.PF.

(Iijima-Ando et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of milt^GD8116 enhances the defects in axon pathology seen when Hsap\MAPT^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF. No neurofibrillary tangles seen in these flies. This enhancement is suppressed upon co-expression of par-1^{dsRNA.Scer\UAS.cMDa}.

(Iijima-Ando et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

par-1^{RNAi.UAS.cMDa}

par-1^{dsRNA.Scer\UAS.cMDa}

par-1^{dsRNA.UAS.cMDa}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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