FB2024_03 , released June 25, 2024
Allele: Dmel\Mp20NIG.4696R
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General Information
Symbol
Dmel\Mp20NIG.4696R
Species
D. melanogaster
Name
FlyBase ID
FBal0220378
Feature type
allele
Associated gene
Dmel\Mp20
Associated Insertion(s)
Carried in Construct
P{NIG.4696R}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
in vitro construct
(Japanese National Institute of Genetics, 2012.5.21, Junion et al., 2007)
Progenitor genotype
Carried in construct
P{NIG.4696R}
Cytology
Description

UASt regulatory sequences drive expression of a 499bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-4696R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Compared with wild-type, an increased number of unfused myoblasts are detected in embryos expressing Mp20NIG.4696R under the control of Scer\GAL4how-24B.

      Compared with wild-type, expression of Mp20NIG.4696R under the control of Scer\GAL4how-24B results in the number of nuclei in muscles DT1 and VA2 decreased by about 2, while a lower decrease is detected in SBM.

      Expression of Mp20NIG.4696R under the control of Scer\GAL4how-24B leads to a reduced rate of embryonic myoblast fusion (relative to wild-type) measured in VA2.

      (Bataillé et al., 2010)

      Embryos expressing Mp20NIG.4696R under the control of Scer\GAL4how-24B show a myoblast fusion defect characterised by rounded cells close to the segment border muscle.

      (Junion et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Mp20NIG.4696R and PaxNIG.18061R under the control of Scer\GAL4how-24B induces a strong decrease in the number of DT1 and VA2 nuclei, two muscles affected by single RNAi knockdowns. In contrast, the phenotype of single knockdowns in SBM is not enhanced by the double knockdowns.

      (Bataillé et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Mp20NIG.4696R
      Name Synonyms
      Secondary FlyBase IDs
        References (3)