Insertion 22bp upstream of the most 5' exon of dl.
axon & eye photoreceptor cell, with Scer\GAL4GMR.PF
axon & eye photoreceptor cell, with Scer\GAL4MT14
Isolated RP2 neuron clones expressing dlGSd447 under the control of Scer\GAL4Act5C.PP (clones generated using the Scer\FLP1eve.RN2 line) show a reduction in dendrite growth at 25-31 hours after egg laying. The distribution of dendrites in the dendritic field is altered: there is a decrease in the number of dendrites targeted to the anterior, posterior, medial and lateral regions of the dendritic field.
SNa motor neurons do not project normally to their muscle targets in embryos expressing dlGSd447 under the control of Scer\GAL4scrt-11-6, but form a discrete bundle alongside the ISN, often bypassing their normal target completely and following a path normally taken by the ISN to muscles located more dorsally.
Expression of dlGSd447 under the control of Scer\GAL4GMR.PF results in robust axon fasciculation and targeting defects in the developing visual system; the lamina layer of the brain is severely depleted and the bundles that project into the medulla are thickened. In addition, the axon terminals often fail to expand. Photoreceptor axons that normally terminate in the lamina project through it to deeper regions of the developing optic lobe. The epithelial and marginal glia are positioned normally in these animals.
The lamina is thin and axon bundles in the medulla are thickened in animals expressing dlGSd447 under the control of Scer\GAL4lz-gal4.
R2-R5 axons show severe mistargeting past the lamina in animals expressing dlGSd447 under the control of Scer\GAL4MT14.
Animals expressing dlGSd447 under the control of Scer\GAL4sca-109-68 do not show axon targeting defects in the developing visual system.