FB2024_03 , released June 25, 2024
Allele: Dmel\dlGSd447
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General Information
Symbol
Dmel\dlGSd447
Species
D. melanogaster
Name
FlyBase ID
FBal0218624
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GSd447
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    Cytology
    Description

    Insertion 22bp upstream of the most 5' exon of dl.

    Allele components
    Component
    Use(s)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Isolated RP2 neuron clones expressing dlGSd447 under the control of Scer\GAL4Act5C.PP (clones generated using the Scer\FLP1eve.RN2 line) show a reduction in dendrite growth at 25-31 hours after egg laying. The distribution of dendrites in the dendritic field is altered: there is a decrease in the number of dendrites targeted to the anterior, posterior, medial and lateral regions of the dendritic field.

    SNa motor neurons do not project normally to their muscle targets in embryos expressing dlGSd447 under the control of Scer\GAL4scrt-11-6, but form a discrete bundle alongside the ISN, often bypassing their normal target completely and following a path normally taken by the ISN to muscles located more dorsally.

    Expression of dlGSd447 under the control of Scer\GAL4GMR.PF results in robust axon fasciculation and targeting defects in the developing visual system; the lamina layer of the brain is severely depleted and the bundles that project into the medulla are thickened. In addition, the axon terminals often fail to expand. Photoreceptor axons that normally terminate in the lamina project through it to deeper regions of the developing optic lobe. The epithelial and marginal glia are positioned normally in these animals.

    The lamina is thin and axon bundles in the medulla are thickened in animals expressing dlGSd447 under the control of Scer\GAL4lz-gal4.

    R2-R5 axons show severe mistargeting past the lamina in animals expressing dlGSd447 under the control of Scer\GAL4MT14.

    Animals expressing dlGSd447 under the control of Scer\GAL4sca-109-68 do not show axon targeting defects in the developing visual system.

    External Data
    Interactions
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    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
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    Xenogenetic Interactions
    Statement
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    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    GSd447GSd447
    dlGSd447
    Name Synonyms
    Secondary FlyBase IDs
      References (3)