FlyBase Allele Report: Dmel\SuUR[UAS.cZa]

General Information

Symbol

Dmel\SuUR^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Zhimulev

FlyBase ID

FBal0218109

Feature type

allele

Associated gene

Dmel\SuUR

Associated Insertion(s)

Carried in Construct

P{UAS-SuUR.Z}

Also Known As

UAS-SuUR, UAS-SuUR⁺

Key Links

Transgenic product class

cDNA

(Zhimulev et al., 2003)

wild_type

(Zhimulev et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhimulev et al., 2003)

wild_type

(Zhimulev et al., 2003)

Mutagen

in vitro construct

(Zhimulev et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-SuUR.Z}

Cytology

Description

Expression of the full ORF and 3'UTR of the SuUR cDNA is governed by UASt sequences.

(Zhimulev et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

SuUR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^AB1

(Yurlova et al., 2009, Kolesnikova et al., 2005)

intercalary heterochromatin, with Scer\GAL4^Sgs3.PD

(Kolesnikova et al., 2005)

polytene chromosome, with Scer\GAL4^Sgs3.PD

(Andreyenkova et al., 2009, Kokoza et al., 2009)

polytene chromosome & salivary gland | conditional - heat sensitive, with Scer\GAL4^Sgs3.PD

(Zhimulev et al., 2003)

Detailed Description

Statement

Reference

Scer\GAL4^Sgs3.PD-mediated expression of SuUR^Scer\UAS.cZa leads to formation of large chromosomal swellings in the intercalary heterochromatin regions, such as the 75C1-2 region.

(Andreyenkova et al., 2009)

Polytene chromosomes in larvae expressing SuUR^Scer\UAS.cZa under the control of Scer\GAL4^Sgs3.PD show swelling in the regions 24D, 25A, 56F, 67A, 67D and 75C.

(Kokoza et al., 2009)

Expression of SuUR^Scer\UAS.cZa under the control of Scer\GAL4^AB1 results in a dramatic reduction in the size of larval salivary gland nuclei compared to controls.

(Yurlova et al., 2009)

Expression of SuUR^Scer\UAS.cZa under the control of Scer\GAL4^AB1 results in the formation of miniature salivary glands. The nuclei of the salivary glands cells are also significantly smaller than normal. Weak points in the polytene chromosomes are still seen in these animals.

Expression of SuUR^Scer\UAS.cZa under the control of Scer\GAL4^Sgs3.PD results in the appearance of swellings in intercalary heterochromatin regions of the salivary gland polytene chromosomes.

(Kolesnikova et al., 2005)

The polytene chromosomes of the salivary glands of SuUR^Scer\UAS.cZa; Scer\GAL4^Sgs3.PD larvae grown at 25'C develop a characteristic pattern of swellings beginning at mid-third instar. By late (wandering) third instar stage, some of these swellings become very large. The size and number of swellings peaks in mid prepupal stages (4-8 hours APF). This phenotype is enhanced at 29'C, suppressed at 18'C and is reversible by switching larvae displaying these swellings at 29'C to 18'C. The number (about 190) and cytological pattern of swellings is highly reproducible, but is completely different from the pattern of chromosome puffs seen in wild-type: swellings arise in chromosome regions where puffs never appear ; the sites of their formation are normally tightly condensed, solid bands and some, such as the region of the Antp and bithorax complexes, are well known examples of silenced regions in intercalary heterochromatin. Analysis of all 190 polytene chromosome bands where swellings appear shows that they represent the regions of intercalary heterochromatin characterized by late replication or underreplication. ^3Huridine labelling shows that, unlike chromosome puffs, these swellings are not sites of transcription.

(Zhimulev et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Sgs3.PD, SuUR^UAS.cZa has polytene chromosome phenotype, suppressible by Df(3L)ED225

(Andreyenkova et al., 2009)

Scer\GAL4^Sgs3.PD, SuUR^UAS.cZa has polytene chromosome phenotype, suppressible | partially by Df(3L)NA34

(Andreyenkova et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^Sgs3.PD, SuUR^UAS.cZa has polytene chromosome phenotype, non-suppressible by Df(3L)ED224

(Andreyenkova et al., 2009)

Scer\GAL4^Sgs3.PD, SuUR^UAS.cZa has polytene chromosome phenotype, non-suppressible by Df(3L)H99

(Andreyenkova et al., 2009)

Scer\GAL4^Sgs3.PD, SuUR^UAS.cZa has polytene chromosome phenotype, non-suppressible by Dp(3;3)NA18

(Andreyenkova et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

No chromosomal swellings occur in the 75C1-2 region in Df(3L)ED225 chromosomes when SuUR^Scer\UAS.cZa is expressed via Scer\GAL4^Sgs3.PD.

Chromosomal swellings of nearly the same size as controls occur in the 75C1-2 region in Df(3L)ED224 or Df(3L)H99 chromosomes when SuUR^Scer\UAS.cZa is expressed via Scer\GAL4^Sgs3.PD.

Chromosomal swellings that are smaller than controls occur in the 75C1-2 region in Df(3L)NA34 chromosomes when SuUR^Scer\UAS.cZa is expressed via Scer\GAL4^Sgs3.PD.

Chromosomal swellings that are the same size as controls occur in the natural and duplicated parts of the 75C1-2 region of Dp(3L)NA18 chromosomes when SuUR^Scer\UAS.cZa is expressed via Scer\GAL4^Sgs3.PD.

(Andreyenkova et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

SuUR^Scer\UAS.cZa

SuUR^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Zhimulev

Secondary FlyBase IDs

References (9)

Report Sections

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