FlyBase Allele Report: Dmel\COX5A[tend]

General Information

Symbol

Dmel\COX5A^tend

Species

D. melanogaster

Name

tenured

FlyBase ID

FBal0217453

Feature type

allele

Associated gene

Dmel\COX5A

Associated Insertion(s)

Carried in Construct

Also Known As

CoVa^tend

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Mandal et al., 2005)

Progenitor genotype

Cytology

Description

A 56bp deletion close to the translational start site of CoVa.

(Mandal et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of COX5A^tend under the control of Scer\GAL4^109(2)80 results in slight shortening of the dendritic arbor in the class IV ddaC neurons in third instar larvae.

(Tsuyama et al., 2017)

Mutant embryos do not show defects in the somatic muscle pattern.

(Tixier et al., 2013)

CoVa^tend mutant eye disc clones exhibit a disruption in cell division as mitotic waves are disrupted, as shown by a failure to incorporate BrdU.

(Mandal et al., 2010)

COX5A^tend homozygous somatic clones induced in a 'minute' background in the eye have a 'glossy' phenotype - the mutant tissue appears smooth due to lack of lens development. Cell division in these clones in the eye disc is normal until the late third instar. However, the second mitotic wave of cell division (posterior to the furrow) fails to occur in mutant cells and at the first mitotic wave (just anterior to the furrow) entry into S-phase is slower than in wild-type. Despite this, ommatidial patterning and differentiation proceed relatively normally in these clones - recruitment to ommatidial pre-clusters occurs normally, although some perturbation of the spacing and orientation of clusters is evident.

FACS analysis of dissociated wing discs containing COX5A^tend homozygous clones shows that a greater fraction of COX5A^tend homozygous cells are in G1 for wild-type cells. Mutant cells are also about 13% larger, on average, than wild-type. BrdU incorporation occurs normally in these clones if they are small (a few cells only), but is largely absent in larger clones. These larger clones are smaller than their adjacent twin-spot.

(Mandal et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

COX5A^tend, Scer\GAL4^109(2)80 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

COX5A^tend has decreased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by p53^11-1B-1/p53^11-1B-1

(Mandal et al., 2010)

COX5A^tend has decreased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by Cul1^EX/Cul1[+]

(Mandal et al., 2010)

COX5A^tend has decreased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by Cul3[+]/Cul3^gft2

(Mandal et al., 2010)

COX5A^tend has decreased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by Prosbeta6[+]/Prosβ6¹

(Mandal et al., 2010)

COX5A^tend has abnormal mitotic cell cycle phenotype, suppressible by p53^11-1B-1

(Mandal et al., 2005)

COX5A^tend has abnormal mitotic cell cycle | somatic clone phenotype, suppressible by SNF4Aγ^KG00325

(Mandal et al., 2005)

Other

Statement

Reference

CoVa[+]/COX5A^tend, Sirup¹/Sirup² has viable phenotype

(Van Vranken et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

COX5A^tend, Scer\GAL4^109(2)80 has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

COX5A^tend, Scer\GAL4^109(2)80 has dendrite | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

COX5A^tend has eye | somatic clone phenotype, suppressible by Pros26[+]/Prosβ6¹

(Mandal et al., 2010)

COX5A^tend has eye | somatic clone phenotype, suppressible by Cul1^EX/lin19[+]

(Mandal et al., 2010)

COX5A^tend has eye | somatic clone phenotype, suppressible by Cul-3[+]/Cul3^gft2

(Mandal et al., 2010)

COX5A^tend has eye | somatic clone phenotype, suppressible by p53[+]/p53^11-1B-1

(Mandal et al., 2010)

COX5A^tend has eye | somatic clone phenotype, suppressible by SNF4Aγ^KG00325

(Mandal et al., 2005)

COX5A^tend has lens | somatic clone phenotype, suppressible by SNF4Aγ^KG00325

(Mandal et al., 2005)

COX5A^tend has eye | somatic clone phenotype, suppressible by p53^11-1B-1

(Mandal et al., 2005)

COX5A^tend has lens | somatic clone phenotype, suppressible by p53^11-1B-1

(Mandal et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the dendritic arbor in class IV ddaC neurons observed in third instar larvae expressing COX5A^tend under the control of Scer\GAL4^109(2)80 can be rescued by co-expression of PPP1R15^Scer\UAS.cMa.

(Tsuyama et al., 2017)

A p53^11-1B-1 background suppresses the cell division disruption found in CoVa^tend mutant eye disc clones.

A Pros26¹ background suppresses the cell division disruption found in CoVa^tend mutant eye disc clones. Interestingly, the glossy adult eye phenotype of CoVa^tend mutant clones is also significantly suppressed.

An ago¹ heterozygous background suppresses the CoVa^tend mutant clone adult eye phenotype.

A lin19^EX or Cul-3^gft2 heterozygous background does not suppress the disruptions in cell division seen in CoVa^tend mutant eye disc clones.

(Mandal et al., 2010)

The 'glossy' eye phenotype due to CoVa^tend homozygous clones is largely suppressed if the clones are also homozygous for SNF4Aγ^KG00325 or if the clones are generated in a p53^11-1B-1 homozygous background. The cell cycle defects seen in CoVa^tend homozygous clones in the eye disc are also suppressed by in these backgrounds.

The 'glossy' eye phenotype due to CoVa^cl-R3 homozygous clones is largely suppressed if the clones are also homozygous for SNF4Aγ^KG00325 or if the clones are generated in a p53^11-1B-1 homozygous background. The cell cycle defects seen in CoVa^cl-R3 homozygous clones in the eye disc are also suppressed by in these backgrounds.

(Mandal et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33839

w^*; P{neoFRT}82B COX5A^tend/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

COX5A^tend

(Monteiro et al., 2023)

CoVa^tend

(Mo et al., 2023, Van Vranken et al., 2014, Tixier et al., 2013)

CoVa^tenured

(Tsuyama et al., 2017)

tend

(Mandal et al., 2005)

Name Synonyms

tenured

Secondary FlyBase IDs

References (7)

Report Sections

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