Deletion generated by recombination between the FRT-bearing P{XP}tupd03613 and PBac{WH}tupf04735 insertions. The deletion removes the entire tup open reading frame (deletion of nucleotides 18,856,481-18,877,346 of chromosome 2L of release 4.2 of the annotated D.melanogaster genome).
gonad | embryonic stage (with tup1)
In tupex4 stage 17 embryos, the testes often exhibit dispersed aggregates of germline niche cells, unlike the single anterior niche position in controls.
The alary muscles in tupex4 mutant embryos are severely malformed and thoracic alary-related muscles are essentially absent.
Homozygous embryos show a duplication of the DA3 muscle (due to a transformation of DA2) in 78% of segments. The attachment sites of the DA3 muscle and the transformed DA2 muscle overlap in the mutant embryos (the attachment sites of DA2 and DA3 do not overlap in wild type). Some partial DA2 to DA3 transformations are seen, resulting in triangular shaped muscles. All dorsal muscles are affected to some extent, with individual muscles often not being recognisable from segment to segment.
tupJQ3 fails to complement tupex4 as judged by the increase in scutellar macrochaetae.
Homozygous clones of tupex4 mutant cells induced during early third instar do not show signs of transformation from notum towards wing hinge as they display normal notum cuticle with notum-like bristles and trichomes.
The bristle phenotype of homozygous clones of tupex4 mutant cells induced during early third instar is complex and position dependent. Extra macrochaetae can arise near most of the extant bristles, being more frequent the more posterior the clones are located on the notum. Macrochaeta duplication is the most common phenotype, except in the scutellar bristle region, where clones often induced the formation of groups of three or more bristles. The clones can also remove extant bristles from several positions. Extra bristles can arise either within or outside tupex4 clones, that is , in the tupex4/+ and even in the +/+ territory. The majority of macrochaeta duplications consists of homozygous tupex4 mutant bristles and rarely tupex4/+ or wild-type bristles. Pairs of tupex4 mutant bristles are never observed in the dorsocentral area. tupex4 mutant macrochaetae never occur in between two dorsocentral bristles. There is no apparent correlation between the size of tupex4 mutant territory and its ability to generate extra bristles within or outside of it. Generally, extra bristles, both within and outwith tupex4 clones, are separated from the extant bristles by several intervening epidermal cells. In contrast, extra bristles at the scutellum can be adjacent to one another.
Homozygous clones in the lateral-posterior region of the notum can result in ectopic structures, such as an ectopic tegula.
Homozygous clones in the wing disc can have smooth borders, and many of these roundish clones partially extrude themselves towards the subjacent epithelial cells. At these stages, the clone cells still maintain their apical connections with the neighbouring wild-type cells.
tupex4 has scutellar bristle | increased number | somatic clone phenotype, suppressible by scase-1
tupex4 has scutellar bristle | increased number | somatic clone phenotype, suppressible by scA
No significant increase in the number of scutellar bristles above the scase-1 background is observed when tupex4 clones are induced in a scase-1 mutant background.
No significant increase in the number of scutellar bristles above the scA background is observed when tupex4 clones are induced in a scA mutant background.
