FB2024_03 , released June 25, 2024
Allele: Dmel\Doa69.short3'UTR.UAS
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General Information
Symbol
Dmel\Doa69.short3'UTR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0216678
Feature type
allele
Associated gene
Dmel\Doa
Associated Insertion(s)
Carried in Construct
P{UAS-Doa.69.short3'UTR}
Key Links
Transgenic product class
wild_type
(Kpebe and Rabinow, 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kpebe and Rabinow, 2008)
Mutagen
in vitro construct
(Kpebe and Rabinow, 2008)
Progenitor genotype
Carried in construct
P{UAS-Doa.69.short3'UTR}
Cytology
Description

UAS regulatory sequences drive expression of the 69kDa Doa isoform, with a shortened 3' UTR (this 3'UTR is found on only a fraction of the 55 and 69kDDa coding transcripts of Doa during the first 4 hours of embryogenesis).

(Kpebe and Rabinow, 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Doa
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Doa69.short3'UTR.Scer\UAS under the control of Scer\GAL4Lsp2.PH results in a slight reduction in viability, with a slightly greater effect on males than females.

      Expression of Doa69.short3'UTR.Scer\UAS under the control of Scer\GAL4GMR.PF results in ommatidial fusion and disorganisation. Small holes are seen in the surface of the ommatidia at high magnification.

      (Kpebe and Rabinow, 2008)

      Expression of Doa69.short3'UTR.Scer\UAS under the control of Scer\GAL4da.G32 results in lethality but with some escapers.

      Expression of Doa69.short3'UTR.Scer\UAS under the control of Scer\GAL4GMR.PF results in a variable eye phenotype (ranging from wild-type with occasional roughness through to roughened eyes and fused and missing ommatidia and pigment loss), depending on the particular P{UAS-Doa.69.short3'UTR} insertion line used and the temperature.

      (Kpebe and Rabinow, 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Doa69.short3'UTR.Scer\UAS
      Doa69.short3'UTR.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)