FB2024_03 , released June 25, 2024
Allele: Dmel\Fas2UAS.cAa
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General Information
Symbol
Dmel\Fas2UAS.cAa
Species
D. melanogaster
Name
FlyBase ID
FBal0215616
Feature type
allele
Associated gene
Dmel\Fas2
Associated Insertion(s)
Carried in Construct
P{UAS-Fas2.A}
Key Links
Transgenic product class
wild_type
(Ashley et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Ashley et al., 2005)
Mutagen
in vitro construct
(Ashley et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-Fas2.A}
Cytology
Description

UAS regulatory sequences drive expression of Fas2 coding sequences.

(Ashley et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fas2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Fas2Scer\UAS.cAa for 12 hours under the control of Scer\GAL4P0.5.Pdf (restricted to the adult stages using Scer\GAL80ts.αTub84B) do not alter the spread of s-LNv projections at dawn (ZT24).

      (Petsakou et al., 2015)

      Overexpression of Fas2Scer\UAS.cAa either presynaptically or postsynaptically (under the control of Scer\GAL4C380 or Scer\GAL4BG487) results in a significant decrease in bouton number. However, when Fas2Scer\UAS.cAa is expressed simultaneously both pre- and post-synaptically, there is a significant increase to >150-230% of the number of synaptic boutons compared with wild-type. This change in bouton number is not attributable to changes in muscle size, which is indistinguishable from wild-type controls.

      There is a dramatic increase in the number of satellites/buds in neuromuscular junctions, 672% over wild-type at both distal and proximal boutons, in flies where Fas2Scer\UAS.cAa is expressed both pre- and post-synaptically (under the control of Scer\GAL4C380 and Scer\GAL4BG487).

      When Fas2Scer\UAS.cAa is expressed both pre- and post-synaptically (under the control of Scer\GAL4C380 and Scer\GAL4BG487), the EJP amplitude is significantly decreased compared to wild-type.

      (Ashley et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Fas2UAS.cAa, Scer\GAL4BG487 has NMJ bouton phenotype, enhanceable by Scer\GAL4futsch-C380/Applsd.UAS

      (Ashley et al., 2005)
      Enhancer of
      Statement
      Reference

      Fas2UAS.cAa/Scer\GAL4BG487 is an enhancer of NMJ bouton phenotype of Applsd.UAS, Scer\GAL4futsch-C380

      (Ashley et al., 2005)
      Suppressor of
      Statement
      Reference

      Fas2UAS.cAa/Scer\GAL4C164 is a suppressor of NMJ bouton phenotype of Appld

      (Ashley et al., 2005)

      Fas2UAS.cAa/Scer\GAL4C57 is a suppressor of NMJ bouton phenotype of Appld

      (Ashley et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Overexpression of Fas2Scer\UAS.cAa in both presynaptic and postsynaptic cells (under the control of Scer\GAL4C164 and Scer\GAL4C57) in Appld mutants leads to a wild-type number of boutons, in contrast to the two-fold increase when Appl levels are normal.

      Overexpression of both Applsd.Scer\UAS and Fas2Scer\UAS.cAa both pre- and post-synaptically (under the control of Scer\GAL4C380 and Scer\GAL4BG487) increase the number of boutons by >400%.

      (Ashley et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Fas2Scer\UAS.cAa
      Fas2UAS.cAa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)