FB2024_03 , released June 25, 2024
Allele: Dmel\Rab32T33N.UASp.YFP
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General Information
Symbol
Dmel\Rab32T33N.UASp.YFP
Species
D. melanogaster
Name
FlyBase ID
FBal0215438
Feature type
allele
Associated gene
Dmel\Rab32
Associated Insertion(s)
Carried in Construct
P{UASp-YFP.Rab32.T33N}
Key Links
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zhang et al., 2007)
missense_variant
(Zhang et al., 2007)
Mutagen
in vitro construct
(Zhang et al., 2007, Zhang et al., 2007)
Progenitor genotype
Carried in construct
P{UASp-YFP.Rab32.T33N}
Cytology
Description

Amino acid replacement: T33N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab32 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Rab32
Tagged with
YFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  epilepsy
      modeled by parabss1
      (Kroll et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing Rab32T33N.UASp.YFP under the control of Scer\GAL4Mef2.247 leads to smaller and rounder mitochondria in adult flight muscles.

      (Chen et al., 2020)

      Animals expressing Rab32T33N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4grh.D4 are viable, no obvious defects in larval dorsal trunk elongation and airway morphology are observed but the larvae are slightly shorter relative to age-matched controls.

      (Wingen et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Scer\GAL4GMR57C10/Rab32T33N.UASp.YFP is a suppressor of bang sensitive phenotype of parabss1

      (Kroll et al., 2015)
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Rab32T33N.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4GMR57C10 partially rescues the bang sensitivity of parabss1/+ flies, but does not rescue the bang sensitivity of hemizygous parabss1 flies or eas2 mutants.

      (Kroll et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rab32T33N.Scer\UAS.P\T.T:Avic\GFP-YFP
      Rab32T33N.UASp.YFP
      ltdT33N.Scer\UAS.P\T.T:Avic\GFP-YFP
      Name Synonyms
      Secondary FlyBase IDs
        References (7)