Amino acid replacement: K717R.
Clones in sensory neurons in adult wing expressing Cul3K717R.Scer\UAS.T:Avic\GFP driven by Ect4ΔARM.Scer\UAS.T:Hsap\MYC do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).
Under light-dark conditions, flies expressing Cul-3K717R.Scer\UAS.T:Avic\GFP under the control of either Scer\GAL41118 or Scer\GAL4tim.PE show reduced morning anticipatory activity. Under constant darkness conditions, the flies either become arrhythmic for locomotor activity or display weak rhythms.
Scer\GAL4ey-OK107/Cul3K717R.UAS.GFP fails to rescue Cul3gft8
Expression of gftK717R.T:Avic\GFP.Scer\UAS under the control of Scer\GAL4OK107 in gft8 mutant clones fails to rescue the axon terminal morphogenesis phenotype.