FB2024_03 , released June 25, 2024
Allele: Dmel\Hs6stRNAi.UAS.cKa
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General Information
Symbol
Dmel\Hs6stRNAi.UAS.cKa
Species
D. melanogaster
Name
FlyBase ID
FBal0212982
Feature type
allele
Associated gene
Dmel\Hs6st
Associated Insertion(s)
Carried in Construct
P{UAS-Hs6st.RNAi.K}
Key Links
Transgenic product class
RNAi_reagent
(Kamimura et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Kamimura et al., 2006)
Mutagen
in vitro construct
(Kamimura et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Hs6st.RNAi.K}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of a 500bp 5' Hs6st cDNA fragment.

(Kamimura et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hs6st
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Alzheimer's disease
      modeled by Hsap\MAPTV337M.UAS
      (Shulman et al., 2011)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Hs6stdsRNA.IR.Scer\UAS.K under the control of Scer\GAL4NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood) display reduced regenerative cell proliferation in the adult posterior midgut upon infection with Pseudomonas entomophila.

      (Takemura and Nakato, 2017)

      Expression of Hs6stdsRNA.IR.Scer\UAS.K (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4GMR.PU has no effect on adult eye appearance.

      (Shulman et al., 2011)

      Expression of Hs6stdsRNA.IR.Scer\UAS.K under the control of Scer\GAL4GMR.PS does not affect development of the eye.

      (Nishihara, 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Hs6stdsRNA.IR.Scer\UAS.K in btl-expressing (tracheal) cells (through the control of Scer\GAL4btl.PS in a Hs2std267 homozygous mutant background completely blocks the formation of the tracheoblast. No such effect is observed when Hs6stdsRNA.IR.Scer\UAS.K is expressed in bnl expressing (non-tracheal) cells (through Scer\GAL4bnl-NP2211) in the same mutant background.

      (Kamimura et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference

      The moderate rough eye phenotype and reduced adult eye size characteristic for flies expressing Scer\GAL4GMR.PU under the control of Hsap\MAPTV337M.Scer\UAS is significantly worsened by co-expression of Hs6stGD3078 (together with UAS-Dicer2 to enhance RNAi efficiency), exacerbating the rough appearance.

      (Shulman et al., 2011)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Hs6stRNAi.IR.UAS.K
      Hs6stRNAi.UAS.cKa
      Hs6stdsRNA.IR.Scer\UAS.K
      Hs6stdsRNA.IR.UAS.K
      Name Synonyms
      Secondary FlyBase IDs
        References (5)