FB2024_03 , released June 25, 2024
Allele: Dmel\Cad99CΔcyt.UAS.EGFP
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General Information
Symbol
Dmel\Cad99CΔcyt.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0212158
Feature type
allele
Associated gene
Dmel\Cad99C
Associated Insertion(s)
Carried in Construct
P{UAS-Cad99C.Δcyt.GFP}
Also Known As
Cad99CΔcyt::GFP
Key Links
Transgenic product class
inframe_deletion
(D'Alterio et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(D'Alterio et al., 2005)
Mutagen
in vitro construct
(D'Alterio et al., 2005)
Progenitor genotype
Cad99CUAS.fl
(D'Alterio et al., 2005)
Carried in construct
P{UAS-Cad99C.Δcyt.GFP}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of Cad99C in which the EGFP tag replaces 256 of the 287 amino acids of the Cad99C cytoplasmic domain.

(D'Alterio et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cad99C
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Cad99CΔcyt.Scer\UAS.T:Avic\GFP-EGFP in the salivary gland under the control of Scer\GAL4fkh.PH results in apical salivary gland membrane expansion. These mutants exhibit an increase in microvilli-like structures and causes apical membranes to protrude into the lateral domain.

      (Chung and Andrew, 2014)

      Expression of Cad99C&cyt.Scer\UAS.T.Avic\GFP-EGFP in follicle cells under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C induces abnormally tall microvilli that fan out from the apical surface, along with enlarged vitelline bodies. This phenotype is seen in both wild-type and Cad99C21-5/Cad99C21-6 backgrounds.

      (D'Alterio et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cad99CΔcyt.Scer\UAS.T.Avic\GFP-EGFP
      Cad99CΔcyt.Scer\UAS.T:Avic\GFP-EGFP
      Cad99CΔcyt.UAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)