Under control conditions, the adulthood-only expression of tra^GD764 under the control of Scer\GAL4^esg-NP7397 (together with Gal80[ts], for the temporal regulation of expression) does not significantly affect the mitotic index in the adult midgut of males and females, as compared to controls.

Expression of tra^GD764 under the control of Scer\GAL4^elav.PLu (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant decrease in virgin female receptivity, as compared to controls.

When wild type female flies are exposed to female flies expressing tra^GD764 under the control of either Scer\GAL4^OK72 or Scer\GAL4^Desat1.PB (which induces masculinization of the pheromone profile) they exhibit a modestly reduced lifespan compared with females exposed to wild type females.

Female flies expressing tra^GD764 under the control of Scer\GAL4^elav-C155 (co-expressed with Dcr-2^Scer\UAS.cDa) exhibit a total loss of the female-specific post-embryonic Ilp7 neurons in the ventral cluster. Embryonic Ilp7 neurons are unaffected. No changes in the Ilp7 neuronal population are seen when tra^GD764 is expressed under the control of Scer\GAL4^Ilp7.PC.

Expression of tra^GD764 under the control of Scer\GAL4^desat1.PB causes the masculinization of female pheromone profiles. As a result, wild-type males show aggression towards females expressing Scer\GAL4^desat1.PB-driven tra^GD764. In contrast, wild-type males do not attack wild-type females.

Females expressing tra^GD764 under the control of Scer\GAL4^elav-C155, unlike wild-type females, show aggression towards bot other females and males. A substantial fraction of wild-type males show aggression towards the females expressing tra^GD764via Scer\GAL4^elav-C155, a behaviour which is not observed towards wild-type females. The pheromone profile of females is unaffected by expression of tra^GD764 via Scer\GAL4^elav-C155. Wild-type males court these females vigorously before transitioning to aggression.

Females expressing tra^GD764 under the control of both Scer\GAL4^desat1.PB and Scer\GAL4^elav-C155 exhibit aggressive behaviour towards wild-type males. These females initiate and dominate most fights. Most of the males who lunge at these females do so prior to or without ever copulating. Females expressing tra^GD764 under the control of both Scer\GAL4^desat1.PB and Scer\GAL4^elav-C155 show a predominantly male pheromone profile.

Females expressing tra^GD764 under the control of Scer\GAL4¹⁴⁰⁷ show a predominantly male pheromone profile.

Wild-type males show aggression towards females expressing tra^GD764 under the control of Scer\GAL4¹⁴⁰⁷. Copulation is not observed for most of these pairs.

Adults expressing tra^GD764 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of tra^GD764 under the control of Scer\GAL4^dsx.KI in XX flies leads to male-like abdominal pigmentation, genitalia and sex combs.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.