FlyBase Allele Report: Dmel\tefu[GD11950]

General Information

Symbol

Dmel\tefu^GD11950

Species

D. melanogaster

Name

FlyBase ID

FBal0210738

Feature type

allele

Associated gene

Dmel\tefu

Associated Insertion(s)

Carried in Construct

P{GD11950}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11950}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11950

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of ataxia telangiectasia

CEA with mei-41^KK101792

(Park et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | adult stage | conditional, with Scer\GAL4^NP5130

(Park et al., 2015)

decreased cell number | adult stage | progressive, with Scer\GAL4^NP5130

(Park et al., 2015)

radiation resistant | cell non-autonomous, with Scer\GAL4^ptc-559.1

(Bilak et al., 2014)

short lived, with Scer\GAL4^NP5130

(Park et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The adulthood-only expression of tefu^GD11950 under the control of Scer\GAL4^esg-NP5130 (in combination with Gal80[ts], for the temporal control of expression) leads to a severe decrease in life-span, as compared to controls; it also results in larger and rounder adult midgut progenitor cells (esg-positive intestinal stem cells and enteroblasts), leads to their disappearance after three weeks of expression, and leads to a significant decrease in the frequency of intestinal stem cells undergoing DNA replication, but does not significantly affect the overall cell density in the adult midgut, the number of intestinal stem cells or the frequency of progenitor cells undergoing DNA replication, as compared to controls. Upon γ-radiation, the adult midgut progenitor cells show a small but evident increase in DNA damage, assessed by pS/TQ and γH2AvD immunofluorescence, as compared to irradiated controls.

(Park et al., 2015)

When larvae expressing tefu^GD11950 under the control of Scer\GAL4^ptc-559.1 are irradiated, less cell death is seen in the wing disc in comparison with irradiated controls. The cells in the A compartment are the first to benefit, but the protective effect is able to reach the P compartment.

(Bilak et al., 2014)

Adults expressing tefu^GD11950 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11950

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^NP5130, tefu^GD11950 has decreased cell number | adult stage | progressive phenotype, enhanceable by mei-41^KK101792/Scer\GAL4^NP5130

(Park et al., 2015)

Enhancer of

Statement

Reference

Scer\GAL4^NP5130/tefu^GD11950 is an enhancer of abnormal DNA repair | adult stage | conditional phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of abnormal cell shape | adult stage phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of decreased cell number | adult stage phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of short lived phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of decreased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Suppressor of

Statement

Reference

tefu^GD11950, Scer\GAL4^nub.PU is a suppressor | partially of visible | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

tefu^GD11950, Scer\GAL4^nub.PU is a suppressor | partially of abnormal size | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^NP5130, tefu^GD11950 has intestinal stem cell of posterior adult midgut epithelium | progressive phenotype, enhanceable by mei-41^KK101792/Scer\GAL4^NP5130

(Park et al., 2015)

Scer\GAL4^NP5130, tefu^GD11950 has enteroblast of posterior adult midgut epithelium | progressive phenotype, enhanceable by mei-41^KK101792/Scer\GAL4^NP5130

(Park et al., 2015)

Enhancer of

Statement

Reference

Scer\GAL4^NP5130/tefu^GD11950 is an enhancer of intestinal stem cell of posterior adult midgut epithelium | conditional phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Scer\GAL4^NP5130/tefu^GD11950 is an enhancer of enteroblast of posterior adult midgut epithelium | conditional phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of enteroblast of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Scer\GAL4^NP5130/tefu^GD11950 is a non-enhancer of adult midgut epithelium phenotype of Scer\GAL4^NP5130, mei-41^KK101792

(Park et al., 2015)

Suppressor of

Statement

Reference

tefu^GD11950, Scer\GAL4^nub.PU is a suppressor | partially of wing phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The small and deformed wings in adult flies expressing His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU are significantly suppressed by co-expression of tefu^GD11950.

(Bayona-Feliu et al., 2017)

The adulthood-only co-expression of mei-41^KK101792 and tefu^GD11950 results in: a more severe increase in γ-radiation-induced DNA damage in adult midgut progenitor cells (esg-positive intestinal stem cells and enteroblasts) compared to either single expression condition; a progressive disappearance of progenitor cells that is comparable to that induced by the expression of mei-41^KK101792 alone but more severe than that induced by the expression of tefu^GD11950 alone; cell shape changes in intestinal progenitors comparable to those induced by the expression of mei-41^KK101792 alone; a decreased cell density in the adult midgut that is comparable to that induced by the expression of mei-41^KK101792 alone; decreases in intestinal stem cell number and DNA replication that are comparable to those induced by the expression of mei-41^KK101792 alone.

(Park et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22502

w¹¹¹⁸; P{GD11950}v22502/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11950

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tefu^GD11950

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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