UASt regulatory sequences drive expression of an inverted repeat.
Expression of spasGD563 under the control of Scer\GAL4ppk.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not lead to any significant defects in axon or dendrite clearance after injury, or dendrite pruning 18h after the onset of pupariation, as compared to controls.
Larval ddaE neurons expressing spasGD563 under the control of Scer\GAL4221 (and Dicer-2, for a more efficient RNAi) do not show significant changes in the number of growing microtubules within the cell body 24h after axotomy, as compared to similarly injured control neurons.
Adults expressing spasGD563 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.