FlyBase Allele Report: Dmel\sds22[GD11788]

General Information

Symbol

Dmel\sds22^GD11788

Species

D. melanogaster

Name

FlyBase ID

FBal0210539

Feature type

allele

Associated gene

Dmel\sds22

Associated Insertion(s)

Carried in Construct

P{GD11788}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11788}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11788

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

follicle cell | somatic clone, with Scer\GAL4^Act.PU

(Grusche et al., 2009)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing disc, with Scer\GAL4^ptc-559.1

(Jiang et al., 2011)

Detailed Description

Statement

Reference

Cells expressing sds22^GD11788 under the control of Scer\GAL4^ptc-559.1 along the anterior-posterior compartment boundary of the wing disc are basally extruded and migrate away from the Scer\GAL4^ptc-559.1 expression domain into the posterior compartment (in contrast to controls, where the cells form a cohesive stripe in the Scer\GAL4^ptc-559.1 expression domain). This results in abnormal apical folding of the disc epithelium along the anterior-posterior compartment boundary.

(Jiang et al., 2011)

Adults expressing sds22^GD11788 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Cells in small clones in the follicular epithelium expressing sds22^GD11788 under the control of Scer\GAL4^Act have a rounded appearance.

(Grusche et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11788

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, sds22^GD11788 has wing disc phenotype, suppressible by Scer\GAL4^ptc-559.1/sqh^GD1695

(Jiang et al., 2011)

Scer\GAL4^ptc-559.1, sds22^GD11788 has wing disc phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/puc^UAS.cMa

(Jiang et al., 2011)

Scer\GAL4^ptc-559.1, sds22^GD11788 has wing disc phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Timp^UAS.cPa

(Jiang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The cell invasion and abnormal folding phenotypes seen in cells expressing sds22^GD11788 under the control of Scer\GAL4^ptc-559.1 along the anterior-posterior compartment boundary of the wing disc are suppressed if the cells are also co-expressing sqh^GD1695.

The cell invasion phenotype seen in cells expressing sds22^GD11788 under the control of Scer\GAL4^ptc-559.1 along the anterior-posterior compartment boundary of the wing disc is suppressed if the cells are also co-expressing puc^Scer\UAS.cMa, but the abnormal apical folding phenotype is not suppressed.

The cell invasion phenotype seen in cells expressing sds22^GD11788 under the control of Scer\GAL4^ptc-559.1 along the anterior-posterior compartment boundary of the wing disc is largely suppressed if the cells are also co-expressing Timp^Scer\UAS.cPa, but the abnormal apical folding phenotype is not suppressed.

(Jiang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v42051

w¹¹¹⁸; P{GD11788}v42051

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11788

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sds22^GD11788

sds22^RNAi

(Pinheiro et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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