UASt regulatory sequences drive expression of an inverted repeat.
The adulthood-only expression of saxGD2546 under the control of Scer\GAL4esg.PU (in combination with Gal80[ts], for temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; this expression does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4dsx.KI (and Gal80[ts]) leads to a significant increase in the number of secretory vesicles, associated with a significant increase in the size of the largest multivesicular body/lysosome, within accessory gland secondary cells compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen.
Expression of saxGD2546 under the control of Scer\GAL4P2.4.Pdf leads to an approximate 60% drop in rhythmicity.
Adults expressing saxGD2546 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 results in duplication of bristle hairs on the notum in 0% or 0-10% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.