Majority of flies expressing mask^GD9632 under the control of Scer\GAL4^Mef2.PU (at 29[o]C) die as late pupae, the adults that manage to eclose are flightless. The myofibrils in indirect flight muscles are thinner than in wild-type controls and the sarcomeres shorter. Ultrastructural defects in the myofibrils range from less-affected (e.g. wavy M-line, some Z disc fragmentation), to severely-affected where all sarcomere structure is lost and Z disc structures form large clumps rather than orderly lines.

Adults expressing mask^GD9632 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in frayed myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 60-70% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% or 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.