UASt regulatory sequences drive expression of an inverted repeat.
Majority of flies expressing maskGD9632 under the control of Scer\GAL4Mef2.PU (at 29[o]C) die as late pupae, the adults that manage to eclose are flightless. The myofibrils in indirect flight muscles are thinner than in wild-type controls and the sarcomeres shorter. Ultrastructural defects in the myofibrils range from less-affected (e.g. wavy M-line, some Z disc fragmentation), to severely-affected where all sarcomere structure is lost and Z disc structures form large clumps rather than orderly lines.
Adults expressing maskGD9632 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in flightless flies.
Expression under the control of Scer\GAL4Mef2.PR results in grossly normal adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in frayed myofibrils in the adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 60-70% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 70-80% or 80-90% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.