FB2024_03 , released June 25, 2024
Allele: Dmel\drdGD3367
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General Information
Symbol
Dmel\drdGD3367
Species
D. melanogaster
Name
FlyBase ID
FBal0209953
Feature type
allele
Associated gene
Dmel\drd
Associated Insertion(s)
Carried in Construct
P{GD3367}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD3367}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD3367
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adults expressing drdGD3367 under the control of either Scer\GAL4DJ626 or Scer\GAL4DJ717 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) lack a peritrophic membrane in the midgut and have reduced defecation rates. Alongside, the presence of holes in the brain indicate signs of neurodegeneration.

      Expressing drdGD3367 under the control of Scer\GAL4hs.PB (with or without a Dicer-2 transgene to enhance RNAi efficiency) hinders peritrophic membrane formation, without affecting defecation rate, when RNAi expression is induced in newly eclosed adults (using a 7 day heat-shock protocol). When RNAi expression is induced during metamorphosis, peritrophic membrane formation is unaffected, but adults are short-lived.

      Expressing drdGD3367 under the control of Scer\GAL4DJ628 does not affect peritrophic membrane formation or adult survival.

      (Conway et al., 2018)

      Adults expressing drdGD3367 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      beltlessGD3367
      drdGD3367
      (Sheahan et al., 2023, Conway et al., 2018)
      lwfGD3367
      Name Synonyms
      Secondary FlyBase IDs
        References (8)