FlyBase Allele Report: Dmel\kst[GD1472]

General Information

Symbol

Dmel\kst^GD1472

Species

D. melanogaster

Name

FlyBase ID

FBal0209802

Feature type

allele

Associated gene

Dmel\kst

Associated Insertion(s)

Carried in Construct

P{GD1472}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1472}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1472

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

photoreceptor cell R8, with Scer\GAL4^GMR.long

(Pojer et al., 2021)

Detailed Description

Statement

Reference

The expression of kst^GD1472 under the control of Scer\GAL4^GMR.long leads to an increase in the ratio of pale vs yellow photoreceptor cell R8.

(Pojer et al., 2021)

Expression of kst^GD1472 results in a very slight posterior roughness in the eye.

(Lee and Thomas, 2011)

Adults expressing kst^GD1472 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing kst^GD1472 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1472

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, kst^GD1472 has visible phenotype, enhanceable by trio^UAS.cBa/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Scer\GAL4^GMR.PFa, kst^GD1472 has visible phenotype, enhanceable by tum^RNAi.UAS.cBa/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, kst^GD1472 has eye phenotype, enhanceable by trio^UAS.cBa/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Scer\GAL4^GMR.PFa, kst^GD1472 has eye phenotype, enhanceable by tum^RNAi.UAS.cBa/Scer\GAL4^GMR.PFa

(Lee and Thomas, 2011)

Enhancer of

Statement

Reference

kst^GD1472, Scer\GAL4^AB1 is an enhancer of embryonic/larval salivary gland phenotype of Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^AB1

(Lee and Thomas, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing both kst^GD1472 and trio^Scer\UAS.cBa under the control Scer\GAL4^GMR.PFa exhibit a strong rough-eye phenotype.

The combined expression of both kst^GD1472 and tum^{dsRNA.Scer\UAS.cBa} via Scer\GAL4^GMR.PFa results in a synergistic interaction and significant disruption of eye development.

Co-expression of Pak^{Scer\UAS.T:Myr1} and kst^GD1472 under the control of Scer\GAL4^AB1 results in the enhancement of the irregular surface of apical membrane and does not rescue the expansion of the salivary gland lumen resulting from the overexpression of Pak^{Scer\UAS.T:Myr1}.

(Lee and Thomas, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v37074