Expressing kis^GD16331 under the control of Scer\GAL4^GMR.PU leads to a glossy, rough eye phenotype.

Expression of kis^GD16331 under the control of Scer\GAL4^da.PU results in a significant increase in the total number of boutons at the neuromuscular junction in third instar larvae.

Expression of kis^GD16331 under the control of Scer\GAL4^how-24B (in the presence of a Dcr-2-expressing line to enhance RNAi efficiency) results in a significant increase in the total number of boutons at the neuromuscular junction in third instar larvae. Expression under the control of Scer\GAL4^elav.PU does not have this effect.

Expression of kis^GD16331 under the control of Scer\GAL4^elav.PU (in the presence of a Dcr-2-expressing line to enhance RNAi efficiency) results in a significant increase in the total number of synaptic branches at the neuromuscular junction in third instar larvae. Expression under the control of Scer\GAL4^how-24B does not have this effect.

Both the frequency of muscle contractions and total crawling distance are significantly reduced compared to wild type in larvae expressing kis^GD16331 under the control of Scer\GAL4^da.PU.

Both the frequency of muscle contractions and total crawling distance are significantly reduced compared to wild type in larvae expressing kis^GD16331 under the control of Scer\GAL4^elav.PU (in the presence of a Dcr-2-expressing line to enhance RNAi efficiency). Expression under the control of Scer\GAL4^how-24B does not have this effect.

Expression of kis^GD16331 under the control of Scer\GAL4^da.G32 or Scer\GAL4^elav-C155 results in late pupal lethality at 25[o]C.

Flies expressing kis^GD16331 under the control of either Scer\GAL4^DJ757, Scer\GAL4^c309 or Scer\GAL4^OK307 show a reduction in climbing ability in the first 10 days after eclosion compared to controls.

Males expressing kis^GD16331 under the control of Scer\GAL4^ey-OK107 show an appropriate learned response in a conditioned courtship suppression assay (similar to wild-type males, they show a significant drop in courtship behaviour in the last 10 minutes of training compared to the first 10 minutes when placed in a courtship chamber with a previously mated (unreceptive) female for 60 minutes). However, the males are deficient in their immediate recall memory, failing to show a decrease in courtship activity compared to sham-trained wild-type flies when tested within 2 minutes of being transferred to a mating chamber with a receptive virgin female.

Flies expressing kis^GD16331 under the control of Scer\GAL4^GMR.PF have disrupted external eye morphology, with the eye having a slightly rough and significantly glassy appearance.

Adults expressing kis^GD16331 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum.