UASt regulatory sequences drive expression of an inverted repeat.
Expression of ITPGD3825 under the control of Scer\GAL4da.Switch.PT in adults fed with RU486 leads to a decrease in the proportion of body fluids when compared to controls.
The phasing of activity peaks of flies expressing ITPGD3825 under the control of Scer\GAL4tim.Scer\UAS is normal under 12 hour light: 12 hour dark conditions. However, the average evening activity is significantly reduced relative to average morning activity. In addition, there is a significant reduction in daytime activity and a significant increase in nighttime activity compared to controls.
Flies expressing ITPGD3825 under the control of Scer\GAL4tim.Scer\UAS remain rhythmic under constant darkness conditions, but there is a slight increase in the length of the period.
Flies expressing ITPGD3825 under the control of Scer\GAL4tim.Scer\UAS show normal sleep profiles and normal total sleep compared to controls.
Adults expressing ITPGD3825 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 40-50% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4pnr-MD237 expression domain.
Co-expression of ITPGD3825 alters the residual activity rhythms of flies expressing PdfGD299 under the control of Scer\GAL4tim.Scer\UAS under constant darkness conditions: activity is clustered in irregular activity bouts and multiple weak periods ranging from 10 to 35 hours are seen.
