FlyBase Allele Report: Dmel\hid[GD1673]

General Information

Symbol

Dmel\hid^GD1673

Species

D. melanogaster

Name

FlyBase ID

FBal0208995

Feature type

allele

Associated gene

Dmel\hid

Associated Insertion(s)

Carried in Construct

P{GD1673}

Also Known As

UAS-hid-RNAi, UAS-hid^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1673}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1673

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile, with Scer\GAL4^nanos.PG

(Czech et al., 2013)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

amnioserosa, with Scer\GAL4^Kr.PM, Scer\GAL4^{Orct2-cald-GAL4}

(Mohseni et al., 2009)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

thorax, with Scer\GAL4^pnr-MD237

(Dietzl et al., 2007)

wing, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

Detailed Description

Statement

Reference

Adults expressing hid^GD1673 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or adult/pharate lethality.

(Schnorrer et al., 2010)

Scer\GAL4^{Orct2-cald-GAL4}, Scer\GAL4^Kr.PM W^GD1673 embryos exhibit a strong, persistent amnioserosa phenotype.

(Mohseni et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of W^GD1673 under the control of Scer\GAL4^Bx-MS1096 results in crinkled wings.

Expression of W^GD1673 under the control of Scer\GAL4^pnr-MD237 causes a mild defective thorax closure phenotype.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1673

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hid^GD1673 has increased cell death phenotype, suppressible by rpr^{UTR.UAS.Tag:HA}/Scer\GAL4^GMR.PF

(Sandu et al., 2010)

Suppressor of

Statement

Reference

hid^GD1673/Scer\GAL4⁴⁷⁷ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ

(Bhogal et al., 2016)

hid^GD1673/Scer\GAL4^{mir-263b-Δ-G4} is a suppressor of visible phenotype of mir-bft^unspecified/mir-bft^Δ263a

(Hilgers et al., 2010)

hid^GD1673/Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA}

(Sandu et al., 2010)

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, hid^GD1673 has lethal phenotype

(Dietzl et al., 2007)

Dcr-2^UAS.cDa, Scer\GAL4^pnr-MD237, hid^GD1673 has lethal phenotype

(Dietzl et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

hid^GD1673 has eye phenotype, suppressible by rpr^{UTR.UAS.Tag:HA}/Scer\GAL4^GMR.PF

(Sandu et al., 2010)

Suppressor of

Statement

Reference

hid^GD1673/Scer\GAL4⁴⁷⁷ is a suppressor of larval multidendritic class IV neuron | third instar larval stage phenotype of Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ

(Bhogal et al., 2016)

hid^GD1673/Scer\GAL4⁴⁷⁷ is a suppressor of dendrite | third instar larval stage phenotype of Scer\GAL4⁴⁷⁷, nanos^RNAi.UAS.WIZ

(Bhogal et al., 2016)

hid^GD1673/Scer\GAL4^{mir-263b-Δ-G4} is a suppressor of interommatidial bristle phenotype of mir-bft^unspecified/mir-bft^Δ263a

(Hilgers et al., 2010)

hid^GD1673/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA}

(Sandu et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritic field coverage defects and loss of terminal branches observed in class IV dendritic arborizing neurons of third instar larvae expressing nos^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4⁴⁷⁷ is suppressed by co-expression of hid^GD1673 RNAi.

(Bhogal et al., 2016)

Expression of W^GD1673 under the control of Scer\GAL4^{mir-263b-Δ-G4} strongly suppresses the loss of interommatidial bristles which is seen in bft^Δ263a/bft^unspecified flies.

(Hilgers et al., 2010)

RNAi-mediated knockdown of W through expression of W^GD1673 under the control of Scer\GAL4^GMR.PF considerably suppressed the cell death phenotype of flies expressing rpr^{UTR.Scer\UAS.T:Ivir\HA1} in the developing eye.

(Sandu et al., 2010)

Coexpression of Dcr-2^Scer\UAS.cDa with W^GD1673, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, results in lethality, while expression of only W^GD1673 with these drivers does not. Coexpression of Dcr-2^Scer\UAS.cDa with W^GD1673 under the control of Scer\GAL4^pnr-MD237 also leads to lethality.

(Dietzl et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

hid^GD1673/Scer\GAL4^GMR.PF rescues hid^GMR.PG

(Sandu et al., 2010)

Comments

RNAi-mediated knockdown of W through expression of W^GD1673 in the developing eye under the control of Scer\GAL4^GMR.PF suppresses W^GMR.PG-induced eye ablation.

(Sandu et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v8269

w¹¹¹⁸; P{GD1673}v8269

v7912

w¹¹¹⁸; P{GD1673}v7912/TM3

Notes on Origin

Discoverer