FlyBase Allele Report: Dmel\Arpc1[GD14085]

General Information

Symbol

Dmel\Arpc1^GD14085

Species

D. melanogaster

Name

FlyBase ID

FBal0208577

Feature type

allele

Associated gene

Dmel\Arpc1

Associated Insertion(s)

Carried in Construct

P{GD14085}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14085}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14085

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Arpc1^GD14085 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in duplication of bristle hairs on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14085

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Arp3^GD12273, Arpc1^GD14085, Scer\GAL4^A3.eya has male sterile phenotype

(Rotkopf et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

Arp3^GD12273, Arpc1^GD14085, Scer\GAL4^A3.eya has seminal vesicle phenotype

(Rotkopf et al., 2011)

Arp3^GD12273, Arpc1^GD14085, Scer\GAL4^A3.eya has spermatid cyst phenotype

(Rotkopf et al., 2011)

Arpc1^GD14085, Scer\GAL4^Mef2.PR has embryonic/larval somatic muscle cell phenotype

(Massarwa et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae co-expressing Arp66B^GD12273 and Sop2^GD14085 under the control of Scer\GAL4^moody.PK show an increased penetration of 3kD fluorescein-dextran into the ventral nerve cord in a permeability assay compared to the dye penetration seen in control larvae.

(Hatan et al., 2011)

Co-expression of Arpc1^GD14085, Arp3^GD12273 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^A3.eya results in a male-sterile phenotype, characterised by spermatogenic arrest at the elongated cyst stage and empty seminal vesicles.

(Rotkopf et al., 2011)

Co-expression of Sop2^GD14085 and Arp66B^GD12273 under the control of Scer\GAL4^btl.PS in embryos does not alter apical F-actin in the trachea.

Co-expression of Sop2^GD14085 and Arp66B^GD12273 under the control of Scer\GAL4^Mef2.PR in embryos results in a muscle fusion phenotype.

(Massarwa et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v42172

w¹¹¹⁸; P{GD14085}v42172/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14085

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Arpc1^GD14085

Sop2^GD14085

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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