UASt regulatory sequences drive expression of an inverted repeat.
Expressing MadGD4121 under the control of Scer\GAL4C587 leads to ectopic transiently amplifying spermatogonia in L3 male gonads.
Expression of MadGD4121 under the control of either Scer\GAL4futsch-C380 or Scer\GAL4C57 results in a reduction in bouton number at the neuromuscular junction (NMJ) in third instar larvae.
Adults expressing MadGD4121 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.
Scer\GAL80ts.αTub84B, MadGD4121, Scer\GAL4da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4da.PU, Scer\GAL80ts.αTub84B, fzrRNAi.UAS.WIZ
Scer\GAL4C587/MadGD4121 is a non-suppressor of abnormal size | recessive | male | third instar larval stage phenotype of Stripjam
Scer\GAL80ts.αTub84B, MadGD4121, Scer\GAL4da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4da.PU, Scer\GAL80ts.αTub84B, fzrRNAi.UAS.WIZ
Scer\GAL4C587/MadGD4121 is a non-suppressor of gonad | male | third instar larval stage phenotype of Stripjam