FlyBase Allele Report: Dmel\Drice[GD12284]

General Information

Symbol

Dmel\Drice^GD12284

Species

D. melanogaster

Name

FlyBase ID

FBal0207360

Feature type

allele

Associated gene

Dmel\Drice

Associated Insertion(s)

Carried in Construct

P{GD12284}

Also Known As

UAS-Drice^RNAi, Drice RNAi, UAS-drICE RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12284}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12284

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant, with Scer\GAL4^ple.PF

(Ortega-Arellano et al., 2013)

hyperplasia | larval stage | conditional, with Scer\GAL4^ci.PU

(Dekanty et al., 2015)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Phenotype Manifest In

wing, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

wing disc anterior compartment | conditional, with Scer\GAL4^ci.PU

(Dekanty et al., 2015)

Detailed Description

Statement

Reference

The expression of Drice^GD12284 under the control of Scer\GAL4^ap-md544 leads to opaque but straight wings, which respectively indicate apoptosis but no growth inhibition during development. Depending on the insertion, additional Dicer-2 expression (for a more efficient RNAi) can induce curled or blistered wings.

Expression under the control of Scer\GAL4^C10 does not affect the overall wing size.

(Shinoda et al., 2019)

Individuals expressing Drice^GD12284 under the control of Scer\GAL4^ci.PU and subjected to ionizing radiation show a significantly enlarged wing disc anterior compartment, associated with basal cell delamination, as compartment to controls.

(Dekanty et al., 2015)

Expression of Drice^GD12284 under the control of Scer\GAL4^ple.PF does not lead to a significant difference in survival or climbing capability as compared to controls under normal conditions; however, these flies exhibit increased resistance to paraquat toxicity, with a significant increase in survival and climbing performance when exposed to paraquat, as compared to control flies exposed to the same treatment.

(Ortega-Arellano et al., 2013)

Adults expressing Drice^GD12284 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12284

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Drice^GD12284, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Suppressor of

Statement

Reference

Drice^GD12284/Scer\GAL4⁴⁷⁷ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of nanos⁵³/nanos¹⁸

(Bhogal et al., 2016)

NOT Suppressor of

Statement

Reference

Drice^GD12284, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Drice^GD12284, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Suppressor of

Statement

Reference

Drice^GD12284/Scer\GAL4⁴⁷⁷ is a suppressor of larval multidendritic class IV neuron | third instar larval stage phenotype of nanos⁵³/nanos¹⁸

(Bhogal et al., 2016)

NOT Suppressor of

Statement

Reference

Drice^GD12284, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(McGurk and Bonini, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritic arborization defects observed in class IV dendritic arborizing neurons of nos¹⁸/nos⁵³ third instar larvae are suppresed by expression of Drice^GD12284 under the control of Scer\GAL4⁴⁷⁷ in the mutant background.

(Bhogal et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v28065