UASt regulatory sequences drive expression of an inverted repeat.
Reduction in Gyc88E through expression of Gyc88EGD11230 in Gyc-89Da cells, under the control of Scer\GAL4Gyc-89Da.1.9 results in larvae that fail to avoid caffeine.
Reduction in Gyc88E through expression of Gyc88EGD11230 in Gyc-89Db cells, under the control of Scer\GAL4Gyc-89Db.3.4 results in larvae that are not attracted to sucrose.
Adults expressing Gyc88EGD11230 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Third instar larvae expressing Gyc88EGD11230 under the control of either Scer\GAL4Gyc-89Da.1.9 or Scer\GAL4Gyc-89Db.3.4 show a defect in the normal hypoxia escape response; mutant larvae which have been allowed to burrow into the food and commence feeding take significantly longer than control larvae to leave the food when exposed to low oxygen concentrations.