UASt regulatory sequences drive expression of an inverted repeat.
Expression of G-iα65AGD12576 under the control of Scer\GAL4Tdc2.PC has no effect on the number of natural synaptopods (motile filopodia-like extensions) or the number of type II boutons at the type II neuromuscular junction in third instar larvae.
Type II neuromuscular junctions show an increase in the number of natural synaptopods in response to octopamine in third instar larvae expressing G-iα65AGD12576 under the control of Scer\GAL4Tdc2.PC, as occurs in wild-type controls.
The number of type I boutons at the neuromuscular junction is normal in third instar larvae expressing G-iα65AGD12576 under the control of either Scer\GAL4Tdc2.PC, Scer\GAL4futsch-C380 or Scer\GAL4BG439.
Flies expressing G-iα65AGD12576 under the control of Scer\GAL4Gr5a.PUn retain a normal preference for 5mM sucrose (over 2mM sucrose) in a 2-choice preference assay.
Adults expressing GαiGD12576 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.