UASt regulatory sequences drive expression of an inverted repeat.
Scer\GAL4tub.PU-mediated expression of EspGD251 in females causes a significant increase in female remating receptivity four days after initial mating, but not at one day after initial mating.
Scer\GAL4tub.PU-mediated expression of EspGD251 in females reduces female fertility over a 10 day assay.
Scer\GAL4tub.PU EspGD251 females mated to wild type males show normal levels of SP in their seminal receptacles at 4 days after mating.
Adults expressing EspGD251 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.