UASt regulatory sequences drive expression of an inverted repeat.
Expression of ETHGD5964 under the control of Scer\GAL4nSyb.PU (in combination with UAS-Dicer2 to increase RNAi efficiency) does not result in a significant change in the latency to sperm ejection in females after the end of copulation.
Expression of ETHGD5964 RNAi under the control of Scer\GAL4ETH.Switch results in severely reduced developmental viability.
Adults expressing ETHGD5964 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.