UASt regulatory sequences drive expression of an inverted repeat.
The expression of Nt5aGD9772 under the control of Scer\GAL4nSyb.PU (together with Dicer-2, for increased RNAi) leads to increased daytime sleep (decreased sleep bout number and increased sleep bout duration, decreased daytime sleep latency) and decreased activity.
Adults expressing CG2277GD9772 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.
Adults expressing CG2277GD9772 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.