Expression of CG16884^GD5391 under the control of Scer\GAL4^ptc-559.1 narrows the L3-L4 intervein region and reduces the anterior crossvein. The anterior crossvein is not ablated, although small gaps are seen in some wings. Expression of CG16884^GD5391 under the control of Scer\GAL4^C-765 results in slightly small wings with some ectopic vein formation, especially in the position of the anterior and posterior crossveins. A reduction in size is also seen when CG16884^GD5391 is expressed under the control of Scer\GAL4^en-e16E. The reduction affects the posterior compartment causing a posterior curvature of the wing. Defects in cuticle formation are also seen. The cuticle of the entire posterior department appears fragile and less pigmented.

Adults expressing CG16884^GD5391 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.