UASt regulatory sequences drive expression of an inverted repeat.
Expression of Ac3GD1799 under the control of Scer\GAL4P2.4.Pdf significantly reduces the response of the M pacemaker neurons in the brain to Pdf. This effect is still seen if expression is restricted to the adult stage by use of the temperature-sensitive Scer\GAL80ts.αTub84B allele.
Expression of Ac3GD1799 under the control of Scer\GAL4P2.4.Pdf does not significantly alter the response of the M pacemaker neurons in the brain to Dh31.
Expression of Ac3GD1799 under the control of Scer\GAL4Mai179 in a Df(2L)DS6/+ background does not significantly alter the response of the E pacemaker neurons in the brain to Pdf.
Flies expressing Ac3GD1799 under the control of Scer\GAL4P2.4.Pdf in a Df(2L)DS6/+ background show a very strong reduction in the morning anticipation peak of circadian locomotor behaviour compared to wild-type flies under light-dark conditions. Under constant darkness conditions, 82% of the flies are arrhythmic.
Adults expressing Ac3GD1799 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Co-expression of Ac3GD1799 and nvyJF03349 under the control of Scer\GAL4P2.4.Pdf reduces the response of M pacemaker neurons in the brain to Pdf to a greater extent than when either Ac3GD1799 or nvyJF03349 are expressed alone.