FlyBase Allele Report: Dmel\Nmnat[UAS.cZa]

General Information

Symbol

Dmel\Nmnat^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zhai

FlyBase ID

FBal0198130

Feature type

allele

Associated gene

Dmel\Nmnat

Associated Insertion(s)

Carried in Construct

P{UAS-Nmnat.Z}

Also Known As

UAS-Nmnat, UAS-dNmnat

Key Links

Transgenic product class

cDNA

(Zhai et al., 2006)

wild_type

(Zhai et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhai et al., 2006)

wild_type

(Zhai et al., 2006)

Mutagen

in vitro construct

(Zhai et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Nmnat.Z}

Cytology

Description

UASt regulatory sequences drive expression of Nmnat (sequences correspond to cDNA clone AT23490).

(Zhai et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nmnat

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Atx-1^UAS.cTa

(Zhai et al., 2008)

ameliorates neurodegenerative disease

modeled by Hsap\ATXN1^82Q.UAS

(Zhai et al., 2008)

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q138.UAS.mRFP1}

(Zhu et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Zhai et al., 2006)

Detailed Description

Statement

Reference

Expression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^elav.PU has no effect on learning and memory in an aversive phototaxis assay at 20 days post eclosion. No locomotor activity defects are seen, as measured by climbing performance and brains do not display any morphological defects.

(Ali et al., 2012)

Overexpression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^elav-C155 has no effect on dendrite outgrowth.

(Wen et al., 2011)

Expression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF protects flies against neuronal degeneration in response to intense light exposure; eyes expressing this transgene show less vacuolization and a higher number of rhabdomeres than wild-type flies in response to 30 days of light exposure.

(Zhai et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nmnat^UAS.cZa, Scer\GAL4^tey-5053A is a suppressor of abnormal neuroanatomy | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

Nmnat^UAS.cZa, Scer\GAL4^VGlut.PD is a suppressor of abnormal locomotor behavior | wandering third instar larval stage phenotype of Scer\GAL4^VGlut.PD, Tmep^KK108183

(Cho et al., 2022)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal learning | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal memory | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy | progressive phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal learning | adult stage phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal memory | adult stage phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy | progressive phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of increased cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Ali et al., 2012)

Scer\GAL4^elav-C155/Nmnat^UAS.cZa is a suppressor of abnormal neuroanatomy phenotype of wts^x1

(Wen et al., 2011)

Nmnat^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Zhai et al., 2008)

Nmnat^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy phenotype of Atx-1^UAS.cTa, Scer\GAL4^GMR.PF

(Zhai et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nmnat^UAS.cZa, Scer\GAL4^tey-5053A is a suppressor of axon | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

Nmnat^UAS.cZa, Scer\GAL4^tey-5053A is a suppressor of embryonic/larval motor neuron | larval stage phenotype of Hsap\SARM1^{SAM.TIR.UAS.Venus}, Scer\GAL4^tey-5053A

(Waller and Collins, 2023)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of brain phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Ali et al., 2012)

Nmnat^UAS.cZa, Scer\GAL4^elav.PU is a suppressor of brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Ali et al., 2012)

Scer\GAL4^elav-C155/Nmnat^UAS.cZa is a suppressor of larval multidendritic class IV neuron phenotype of wts^x1

(Wen et al., 2011)

Scer\GAL4^elav-C155/Nmnat^UAS.cZa is a suppressor of dendrite phenotype of wts^x1

(Wen et al., 2011)

Nmnat^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor of rhabdomere of eye photoreceptor cell phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Zhai et al., 2008)

Nmnat^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Zhai et al., 2008)

Nmnat^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor of rhabdomere of eye photoreceptor cell phenotype of Atx-1^UAS.cTa, Scer\GAL4^GMR.PF

(Zhai et al., 2008)

Scer\GAL4^GMR.PF/Nmnat^UAS.cZa is a suppressor | partially of rhabdomere phenotype of rdgA^unspecified

(Zhai et al., 2006)

Scer\GAL4^GMR.PF/Nmnat^UAS.cZa is a suppressor | partially of eye photoreceptor cell phenotype of rdgA^unspecified

(Zhai et al., 2006)

Scer\GAL4^GMR.PF/Nmnat^UAS.cZa is a suppressor of rhabdomere phenotype of trp^P365

(Zhai et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Toll-6-D42/Nmnat^UAS.cZa is a non-suppressor of mitochondrion | somatic clone | third instar larval stage phenotype of milt^33-853

(Kitay et al., 2013)

Scer\GAL4^Toll-6-D42/Nmnat^UAS.cZa is a non-suppressor of motor neuron | somatic clone | third instar larval stage phenotype of milt^33-853

(Kitay et al., 2013)

Scer\GAL4^GMR.PF/Nmnat^UAS.cZa is a non-suppressor of eye photoreceptor cell phenotype of trp^P365

(Zhai et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The anterograde mitochondria transport defects of single cell milt^33-853 larval motor neuron clones are not rescued by expression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^D42.

Expression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^elav-C155 protects both wild-type and milt^33-853 motor neuron clones from degeneration after axotomy at 16 hours after crushing.

(Kitay et al., 2013)

Whereas wts^x1 ddaC MARCM clones show a simplifed dendritic arbor with significantly reduced numbers of terminal branches, wts^x1 clones overexpressing Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^elav-C155 elaborate dendrites with terminal branch numbers that do not differ statistically from wild-type controls. A similar rescue is seen in class IV neurons.

(Wen et al., 2011)

Co-expression of Nmnat^Scer\UAS.cZa suppresses the rhabdomere degeneration phenotype induced by Scer\GAL4^GMR.PF>Atx-1^Scer\UAS.cTa.

(Zhai et al., 2008)

The loss of rhabdomere phenotype of rdgA^unspecified mutants is significantly rescued by either Nmnat^Scer\UAS.cZa or Nmnat^WR.Scer\UAS, under the control of Scer\GAL4^GMR.PF. The size of vacuoles that are present in the retina and photoreceptors of rdgA^unspecified mutants are reduced by expression of Nmnat^WR.Scer\UAS, but not Nmnat^Scer\UAS.cZa.

The number of rhabdomeres per ommatidium is significantly rescued in trp^P365 flies by expression of either Nmnat^Scer\UAS.cZa or Nmnat^WR.Scer\UAS under the control of Scer\GAL4^GMR.PF. However, the vacuolarization that occurs in trp^P365 retinae and photoreceptors is not suppressed by either transgene.

(Zhai et al., 2006)

Xenogenetic Interactions

Statement

Reference

Expression of Nmnat^Scer\UAS.cZa suppresses the learning and memory deficits seen in 20 day old flies expressing Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^elav.PU. The climbing defects are suppressed to near wild type levels. The brain vacuolisation seen at 20 days post eclosion is almost completely suppressed.

Expression of Nmnat^Scer\UAS.cZa suppresses the learning and memory deficits seen in 20 day old flies expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PU. The climbing defects are also suppressed to near wild type levels. The brain vacuolisation seen at 20 days post eclosion is almost completely suppressed and fewer apoptotic cells are seen in the midbrain.

(Ali et al., 2012)

The rough eye and rhabdomere phenotypes of Scer\GAL4^GMR.PF>Hsap\ATXN1^82Q.Scer\UAS are partially suppressed by co-expression of Nmnat^Scer\UAS.cZa.

(Zhai et al., 2008)

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR.PF/Nmnat^UAS.cZa rescues Nmnat¹

(Zhai et al., 2006)

Scer\GAL4^Act5C.PI/Nmnat^UAS.cZa rescues Nmnat¹

(Zhai et al., 2006)

Comments

Post-synaptic expression of Nmnat^Scer\UAS.cZa under the control of Scer\GAL4^21-7 is sufficient to rescue the dendritic phenotypes observed in Nmnat^Δ4790-2 ddaC clones, demonstrating a cell-autonomous function for Nmnat in the proper maintenance of class IV dendrites.

(Wen et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39699

y¹ w^*; P{UAS-Nmnat.Z}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nmnat^Scer\UAS.cZa

Nmnat^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zhai

Secondary FlyBase IDs

References (15)

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