UASt regulatory sequences drive expression of Mmus\Nkx2-5 tagged with Tag:FLAG.
At 18[o]C, overexpression of Mmus\Nkx2-5Scer\UAS.T:Zzzz\FLAG in the eye, under the control of Scer\GAL4GMR.PU, results in variable levels of eye defects. Expression in the wing, under the control of Scer\GAL4vg.PU results in wing defects. The penetrance is complete at 25[o]C.