FlyBase Allele Report: Dmel\Med[UAS.cSa]

General Information

Symbol

Dmel\Med^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Stefancsik

FlyBase ID

FBal0196303

Feature type

allele

Associated gene

Dmel\Med

Associated Insertion(s)

Carried in Construct

P{UAS-Med.S}

Also Known As

UAS-Med, UAS.Med

Key Links

Transgenic product class

cDNA

(Sutherland et al., 2003)

wild_type

(Sutherland et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sutherland et al., 2003)

wild_type

(Sutherland et al., 2003)

Mutagen

in vitro construct

(McCabe et al., 2004, Sutherland et al., 2003, Marquez et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Med.S}

Cytology

Description

Expression of the Medea cDNA (AF027729) is under the control of UASt sequences.

(Sutherland et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Med

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^A9

(Marquez et al., 2001)

visible, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

visible | adult stage, with Scer\GAL4^ey.PU

(Li et al., 2021)

visible | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2021)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Li et al., 2021)

eye, with Scer\GAL4^GMR.PU

(Li et al., 2021)

leg, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

leg | ectopic, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

wing, with Scer\GAL4^A9

(Marquez et al., 2001)

wing, with Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

wing, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

wing vein, with Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

wing vein | ectopic, with Scer\GAL4^A9

(Fischer et al., 2012, Marquez et al., 2001)

wing vein | ectopic, with Scer\GAL4^ptc-559.1

(Marquez et al., 2001)

Detailed Description

Statement

Reference

Expression of Med^Scer\UAS.cSa with Scer\GAL4^A9 results in ectopic wing vein tissue and altered wing shape.

(Fischer et al., 2012)

Expression of Med^Scer\UAS.cSa using Scer\GAL4^salm-459.2 has no severe effects on wing growth or patterning.

Expression of Med^Scer\UAS.cSa using Scer\GAL4^nub-AC-62 causes alterations in the vein pattern and wing size.

(Barrio et al., 2007)

When expression is driven by Scer\GAL4^ptc-559.1, ectopic vein tissue appears between wing veins L3 and L5. The distance between L3 and L5 appears reduced. Wings are 11% larger than wild type. Ectopic legs have abnormally short and wide tibia that lead to duplicated tarsi on one or more segments. Ectopic legs show severe bristle overgrowth. Alternatively, legs may be truncated. When expression is driven by Scer\GAL4^A9, wings are enlarged by approximately 22%. Ectopic wing veins also develop.

(Marquez et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Med^UAS.cSa, Scer\GAL4^A9 is a suppressor | partially of visible phenotype of Scer\GAL4^A9, Snoo^UAS.cTa

(Takaesu et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Med^UAS.cSa, Scer\GAL4^A9 has wing vein | ectopic phenotype, suppressible | partially by fuss^UAS.C, Scer\GAL4^A9

(Fischer et al., 2012)

NOT suppressed by

Statement

Reference

Med^UAS.cSa, Scer\GAL4^nub-AC-62 has wing phenotype, non-suppressible by Snoo^M67, Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

Med^UAS.cSa, Scer\GAL4^nub-AC-62 has wing vein phenotype, non-suppressible by Snoo^M67, Scer\GAL4^nub-AC-62

(Barrio et al., 2007)

Suppressor of

Statement

Reference

Med^UAS.cSa, Scer\GAL4^salm-459.2 is a suppressor of wing phenotype of Scer\GAL4^salm-459.2, Snoo^M67

(Barrio et al., 2007)

Med^UAS.cSa, Scer\GAL4^salm-459.2 is a suppressor of wing vein phenotype of Scer\GAL4^salm-459.2, Snoo^M67

(Barrio et al., 2007)

Med^UAS.cSa, Scer\GAL4^nub-AC-62 is a suppressor of wing vein phenotype of Scer\GAL4^nub-AC-62, Snoo^M67

(Barrio et al., 2007)

Med^UAS.cSa, Scer\GAL4^A9 is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Snoo^UAS.cTa

(Takaesu et al., 2006)

Med^UAS.cSa, Scer\GAL4^A9 is a suppressor | partially of wing vein phenotype of Scer\GAL4^A9, Snoo^UAS.cTa

(Takaesu et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of fuss^Scer\UAS.C restores wign shape and vein patterning of Med^Scer\UAS.cSa, Scer\GAL4^A9 wings to an almost wild type mode, with only slight failure of complete outgrowth of L5 and the posterior crossvein.

(Fischer et al., 2012)

Co-expression of Med^Scer\UAS.cSa suppresses the wing phenotypes caused by Scer\GAL4^salm-459.2-mediated expression of Snoo^M67, giving rise to wings of almost normal size and pattern.

Co-expression of Snoo^M67 does not suppress the Med^Scer\UAS.cSa, Scer\GAL4^nub-AC-62 wing phenotype. However, the vein phenotype seen in Snoo^M67, Scer\GAL4^nub-AC-62 wings is suppressed by co-expression of Med^Scer\UAS.cSa.

(Barrio et al., 2007)

Co-expression of Med^Scer\UAS.cSa partially rescues the phenotype caused by expression of Snoo^Scer\UAS.cTa under the control of Scer\GAL4^A9; wing size is rescued to wild type and the penetrance of the multiple wing vein defects is reduced to 28%.

(Takaesu et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Med^UAS.cSa/Scer\GAL4^elav.PLu rescues Med^C246/Med³

(McCabe et al., 2004)

Fails to rescue

Scer\GAL4^G14/Med^UAS.cSa fails to rescue Med^C246/Med³

(McCabe et al., 2004)

Comments

Expression of Med^Scer\UAS.cSa under the control of Scer\GAL4^G14 in the muscles does not rescue neuromuscular junction size in Med³/Med^C246 mutants. Expression of Med^Scer\UAS.cSa under the control of Scer\GAL4^elav.PLu in the neurons rescues neuromuscular junction size in Med³/Med^C246 mutants.

(McCabe et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Med^Scer\UAS.cAa

Med^Scer\UAS.cSa

Med^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Stefancsik

Secondary FlyBase IDs

FBal0156260
FBal0123136

References (9)

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