A 3kb SacII-XbaI fragment has been replaced with an equivalent fragment from a moody cDNA with prespliced fourth introns, which corresponds to the RB, or beta, transcript.
Flies that express moody+tgenβ in a Df(1)moody-Δ17 background show increased cocaine sensitivity, as measured by their behaviour in response to the drug.
moody+tgenβ partially rescues Df(1)moody-Δ17
moody+tgenβ partially rescues Df(1)moody-Δ17
The moody+tgenβ transgene rescues the viability of Df(1)moody-Δ17 flies, but not the increased cocaine sensitivity. This transgene restores the blood-brain barrier in all Df(1)moody-Δ17 flies.
Expression of the moody+tgenβ transgene provides significant rescue of the blood-brain barrier formation defect of Df(1)moody-Δ17 mutants.