A 3kb SacII-XbaI fragment has been replaced with an equivalent fragment from a moody cDNA with prespliced fourth introns, which corresponds to the RA, or alpha) transcript.
Flies that express moody+tgenα in a Df(1)moody-Δ17 background show increased cocaine sensitivity, as measured by their behaviour in response to the drug.
moody+tgenα partially rescues Df(1)moody-Δ17
moody+tgenα partially rescues Df(1)moody-Δ17
Expression of the moody+tgenα transgene restores viability of 20-25% of Df(1)moody-Δ17 flies but does not rescue the increased sensitivity to cocaine. This transgene restores the blood-brain barrier in all Df(1)moody-Δ17 flies.
Expression of the moody+tgenα transgene provides significant rescue of the blood-brain barrier formation defect of Df(1)moody-Δ17 mutants.