In control stage 15 embryos, the anterior and posterior lateral trunks (Lta and Ltp, respectively) from adjacent segments are joined. In contrast, gaps occur between the Lta and Ltp in stage 15 embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4btl.PS.
By stage 15, in control embryos, tracheal ganglionic cells and branches enter the ventral nerve cord (VNC) toward the central nervous system (CNS) midline. In contrast, three ganglionic branch (Gb) phenotypes are observed in in embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4btl.PS in all tracheal cells: (1) Gbs do not reach the CNS midline appearing truncated; (2) Gbs show breaks; and (3) Gbs are absent from ventral positions
Embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4btl.PS show the aberrant presence of dorsal branch cells in adjacent tracheal metameres instead of near the dorsal midline as in control embryos.
Fas2-positive longitudinal axons aberrantly cross the central nervous system midline and there are breaks in the longitudinal connectives in stage 16 embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PLu.
robo-positive axons cross the central nervous system midline in stage 16 embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PLu.
Longitudinal glia inappropriately occupy positions at the central nervous system midline in stage 12 embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4gcm.PP.
Embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PLu have a normal number of glia in the ventral nerve cord at both stage 12 and stage 15. Embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4gcm.PP have a normal number of glia in the ventral nerve cord at stage 12, but the number of glia is reduced compared to controls at stage 15.
robo-positive glia are misplaced medially in stage 12/13 embryos expressing jingdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4gcm.PP.
Longitudinal fascicles fuse into a single tract at the midline in stage 16 embryos co-expressing jingdsRNA.Scer\UAS.WIZ and XNPdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PLu.
