When zfh1^Scer\UAS.cLa is expressed weakly in neuroblasts and ganglion mother cells and strongly in postmitotic neurons, driven by Scer\GAL4^elav-C155, the ISNb becomes misrouted into adjacent hemisegments and there are disrupted interneuronal projections. At least 3 out of 12 neuroblast lineages show ectopic motor neuron projections. When this transgene is expressed strongly in neuroblasts and more weakly in ganglion mother cells and neurons, driven by Scer\GAL4^s.gcm, motor nerves become thicker and too disorganized to identify and interneuronal projections are severely perturbed.

Expression of zfh1^Scer\UAS.cLa in the 7-3 neuroblast lineage, driven by Scer\GAL4^eg-Mz360, results in the redirection of axons from EW1-EW3 interneurons so that these axons travel laterally out of the CNS instead of medially across the posterior commissure.

Expression of zfh1^Scer\UAS.cLa in the dMP2 neuron, driven by Scer\GAL4^22B, causes the dMP2 neuron to redirect its axon laterally out of the CNS into the lateral muscle field.