Imprecise excision, removing ~37kb, starting at the 158bp P{lacW}EcR11 insertion site and continuing to 92bp upstream of the EcR-B transcription start site.
98% of EcR94/EcRM554fs mutants fail to pupariate. Instead, the internal tissues of these animals become unstable and undergo necrosis. In the 2% of mutants that pupariate, eversion of the anterior spiracles occurs in an abnormal position, the pupal case is sometimes misshapen and after 24 hours, pupae undergo desiccation.
Selected as: F2 progeny with white eyes that cannot complement the EcRM554fs mutation for viability.