Expression of cryScer\UAS.cDa, under the control of Scer\GAL4tim.PE in flies exposed to constant darkness does not alter the period length. The free-running period for these flies is the same as for controls. cryb flies expressing cryScer\UAS.cDa, under the control of Scer\GAL4tim.PE generate a reduced phase-shift response to a short light-pulse
Scer\GAL4tim.PE, cryUAS.cDa, cryb has abnormal circadian rhythm phenotype, suppressible by 5-HT1BRNAi.UAS, Scer\GAL4tim.PE
Increasing cry levels through cryScer\UAS.cDa expression, eliminates the strong behavioural rhythms in constant bright light observed in cryb heterozygous flies overexpressing 5-HT1BScer\UAS.cYa under the control of Scer\GAL4tim.PE.
Scer\GAL4tim.PE/cryUAS.cDa rescues cryb
Overexpression of cryScer\UAS.cDa, under the regulation of Scer\GAL4tim.PE rescues the cryb phenotype, with the flies becoming desynchronised and arrhythmic after 1 or 2 days.