Dissel, S., Codd, V., Fedic, R., Garner, K.J., Costa, R., Kyriacou, C.P., Rosato, E. (2004). A constitutively active cryptochrome in Drosophila melanogaster. Nat. Neurosci. 7(8): 834--840.
FlyBase ID
FBrf0179188
Publication Type
Research paper
Abstract
Light-activated cryptochrome (CRY) regulates circadian photoresponses in Drosophila melanogaster. Removing the carboxy (C) terminus to create CRYDelta produces, in yeast, a light-independent, constitutively active form. Here we show that flies overexpressing CRYDelta have a longer free-running period of locomotor activity, as well as altered cycling kinetics of the clock proteins timeless (TIM) and period (PER). Moreover, at the cellular level, they show a reduction in the level of TIM and in the nuclear localization of TIM and PER in two significant clusters of behavioral pacemaker cells: the large and the small ventral lateral neurons (LN(v)s). These effects are similar to those seen in wild-type flies under continuous light and suggest a regulatory role for the C terminus of CRY on the photosensitive, photolyase-like part of the protein.
