mesothoracic ventral neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS
metathoracic ventral thoracic neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS
prothoracic ventral neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS
Adults in which EcRB2-ΔC655.W650A.Scer\UAS has been expressed under the control of Scer\GAL4hs.PU using a 25 minute heat shock during the wandering third larval instar have reduced total body triglyceride levels (normalised to total body protein) compared to controls.
Thoracic ventral neurosecretory cells that express EcRB2-ΔC655.W650A.Scer\UAS under the control of Scer\GAL4Fmrf.PS are of normal size and larval morphology at the start of metamorphosis. However, these mutants show defective neuronal remodelling during metamorphosis. The axons show much lower levels of pruning compared to wild-type cells and show no filopodia during the pruning phase. EcRB2-ΔC655.W650A.Scer\UAS-expressing neurons start extending filopodia after 24 hours APF from the neurohemal organ and in the growth zone below. As this happens, the remnant neurohemal organ partially fragments, resulting in an adult-like arbor with clumped varicosities and a dense centre. Effects on adult axon outgrowth are relatively mild, with axonal arbors slighlty reduced compared to controls.