FB2024_03 , released June 25, 2024
Allele: Dmel\EcRB2-ΔC655.W650A.UAS
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General Information
Symbol
Dmel\EcRB2-ΔC655.W650A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0189994
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences drive expression of a mutated EcR B2 isoform (carries the amino acid replacement W650A) that is truncated after residue 655.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

mesothoracic ventral neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS

metathoracic ventral thoracic neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS

prothoracic ventral neurosecretory neuron & axon, with Scer\GAL4FMRFa.PS

Detailed Description
Statement
Reference

Adults in which EcRB2-ΔC655.W650A.Scer\UAS has been expressed under the control of Scer\GAL4hs.PU using a 25 minute heat shock during the wandering third larval instar have reduced total body triglyceride levels (normalised to total body protein) compared to controls.

Thoracic ventral neurosecretory cells that express EcRB2-ΔC655.W650A.Scer\UAS under the control of Scer\GAL4Fmrf.PS are of normal size and larval morphology at the start of metamorphosis. However, these mutants show defective neuronal remodelling during metamorphosis. The axons show much lower levels of pruning compared to wild-type cells and show no filopodia during the pruning phase. EcRB2-ΔC655.W650A.Scer\UAS-expressing neurons start extending filopodia after 24 hours APF from the neurohemal organ and in the growth zone below. As this happens, the remnant neurohemal organ partially fragments, resulting in an adult-like arbor with clumped varicosities and a dense centre. Effects on adult axon outgrowth are relatively mild, with axonal arbors slighlty reduced compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
EcRB2-ΔC655.W650A.Scer\UAS
EcRB2-ΔC655.W650A.UAS
Name Synonyms
Secondary FlyBase IDs
    References (10)