Amino acid replacement: T?I. Nucleotide substitution: C?T. The mutation is within exon 9.
C17951440T
C?T
T203I | Sh-PA; T248I | Sh-PB; T236I | Sh-PC; T203I | Sh-PD; T248I | Sh-PE; T248I | Sh-PF; T203I | Sh-PH; T236I | Sh-PI; T236I | Sh-PJ; T196I | Sh-PK; T236I | Sh-PL; T236I | Sh-PM; T248I | Sh-PQ; M1I | Sh-PS; T248I | Sh-PT
T?I
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator based on author statement (see Figure 3 of FBrf0184967)
Mutant flies show a reduced amount of daily sleep compared to controls.
Mutant flies have normal mushroom body morphology.
The average amount of daily sleep in Shmns flies is reduced compared to wild type (584 +/- 13 minutes for the mutant flies versus 965 +/- 15 minutes for wild type).
The EC[[50]] value for the volatile anesthetics isoflurane and sevoflurane are significantly increased in the mutant flies compared to wild type.
Shmns flies respond to 5-HTP treatment with an increase in daily sleep and a significant reduction in night-time sleep bout length, compared to controls where daily sleep is increased but there is no change in night-time sleep bout length.
Mutant flies exhibit a decrease in the daily sleep amount. This is mainly due to a decrease in the duration of sleep episodes rather than in their number. Mutant flies maintain a rhythmic modulation of locomotor activity with a period of about 24h. the penetrance of this phenotype is not complete. When sleep deprived, mutants (like wild-type) show an increase in sleep duration and intensity. However the increase in intensity is not as marked as seen in wild-type. Mutant flies show no obvious developmental or other behavioural phenotype. However the performance of mutant flies in complex stimulus or thermal response tests is considerably less reduced after sleep deprivation compared to wild-type. Mutant animals exhibit a transient shaking of the legs and scissoring of the wings when recovering from diethyl ether anaesthesia. This phenotype is dominant and persists in amputated legs. It is not seen when intact flies are exposed to chloroform, haloethane, isoflurane or enflurane. Mutants have a shorter lifespan than controls.
