The PBac{RB}csule00797 insertion is located within exon 2. This results in the production of a truncated protein, due to the presence of multiple in-frame stop codons. This generates a protein without the methyltransferase domain.
Progeny obtained from homozygous csule00797 mothers are sterile and agametic. Embryos from csule00797 mothers are devoid of pole cells.
Homozygous csule00797 animals are completely viable and survive to adulthood.
csule00797 mutants display no obvious locomotor defects at either larval or adult stages. There is no obvious degeneration or disorganisation of thoracic musculature in adult csule00797 mutants.
csule00797 mutant testes contain fewer sperm bundles than in wild-type, and display a dramatic loss of organisation among the sperm bundles. The average bundle contains far fewer spermatid nuclei than in the wild-type control. The seminal vesicles of csule00797 males are almost completely devoid of sperm.
Mutant csule00797 females are fertile, albeit with slightly reduced fecundity. When crossed to wild-type males, approximately 65% of the embryos hatch into larvae that develop normally into adult flies. However, all of these flies are agametic, and therefore sterile. The remaining 35% of the embryos display a variety of segmentation defects, as compared to wild-type. All embryos are completely devoid of pole cells.
csule00797 is a non-enhancer of lethal | pupal stage phenotype of Art7NIG.9882R, Scer\GAL4da.G32
SmnE33, csule00797 has lethal | pupal stage phenotype
csule00797 is rescued by Scer\GAL4nanos.PG/csulUAS.Tag:MYC
Pole cells formed in csule00797 mutant embryos that express csulScer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.PG are functional. At 22oC, the rescue frequency is approximately 30%. Incubation at 29oC increases the rescue to approximately 70%.
