FlyBase Allele Report: Dmel\dlt[RNAi.UAS]

General Information

Symbol

Dmel\dlt^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0180174

Feature type

allele

Associated gene

Dmel\dlt

Associated Insertion(s)

Carried in Construct

P{UAS-dlt.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Pielage et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Pielage et al., 2003)

Mutagen

in vitro construct

(Pielage et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-dlt.RNAi}

Cytology

Description

An inverted repeat of the dlt open reading frame (bases 168-854) is separated by a w intron and placed under the control of UAS sequences.

(Pielage et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dlt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell size, with Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

decreased cell number, with Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

increased cell death, with Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

Phenotype Manifest In

crossvein, with Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

eye, with Scer\GAL4^ey.PH

(Pielage et al., 2003)

wing, with Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

wing hair | increased number, with Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

Detailed Description

Statement

Reference

Expression of dlt^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH causes small eyes. Expression of dlt^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 in the wing disc epithelium causes a reduction in cell number; wild-type wings have about eighteen cells separating the longitudinal veins L3 and L4, while there are only about 16 cells, of slightly increased cell size, in the corresponding place of Scer\GAL4^ptc-559.1>dlt^{dsRNA.Scer\UAS} wings. Expression of higher levels of dlt^{dsRNA.Scer\UAS}, still driven by Scer\GAL4^ptc-559.1, results in a further reduction in cell number, a loss of crossvein 1 and a multiple wing hair phenotype. Occasionally, necrotic cells are observed. The removal of one copy of dlt enhances this phenotype.

(Pielage et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has decreased cell number phenotype, enhanceable by CycE^UAS.cLa, Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has decreased cell number phenotype, non-enhanceable by CycA^UAS.Tag:HA, Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has decreased cell number phenotype, non-enhanceable by Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has decreased cell number phenotype, non-enhanceable by Scer\GAL4^ptc-559.1/stg^UAS.cNa

(Pielage et al., 2003)

Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has decreased cell number phenotype, non-enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1

(Pielage et al., 2003)

Phenotype Manifest In

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has wing vein L3 phenotype

(Pielage et al., 2003)

BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has wing blade dorsal compartment phenotype

(Pielage et al., 2003)

BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1, dlt^RNAi.UAS has wing blade ventral compartment phenotype

(Pielage et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of dlt^{dsRNA.Scer\UAS} and CycE^Scer\UAS.cLa, both under the control of Scer\GAL4^ptc-559.1, results in a dramatic loss of wing cells. Expression of dlt^{dsRNA.Scer\UAS} in a CycE^unspecified heterozygous mutant background causes only a very moderate decrease in the number of wing cells. Coexpression of dlt^{dsRNA.Scer\UAS} with either CycA^{Scer\UAS.T:Ivir\HA1} or stg^Scer\UAS.cNa (all driven by Scer\GAL4^ptc-559.1) has little or no effect on the dlt^{dsRNA.Scer\UAS} wing cell phenotype.

(Pielage et al., 2003)

Xenogenetic Interactions

Statement

Reference

Coexpression of dlt^{dsRNA.Scer\UAS}, driven by Scer\GAL4^ptc-559.1, and BacA\p35^Scer\UAS.cHa does not alter the number of surviving cells in the wing. However, the dorsal and ventral wing blades are often detached in these mutants and the L3 vein appears broader.

(Pielage et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transfected into flies to study the the phenotypic consequences of dsRNA interference (RNAi) of the dlt gene.

(Pielage et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dlt^RNAi.UAS

dlt^{dsRNA.Scer\UAS}

dlt^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close